KOYAMA Hiroyuki

J-GLOBAL         Last updated: Dec 27, 2018 at 16:36
KOYAMA Hiroyuki
Gifu University
Faculty of Applied Biological Sciences
Research funding number


Molecular Biology and Genetics in Plant Tolerance to Acid Soil stress/Acid soil stress is the most serious stress factor that limits world food production. My research is focusing to clarify complex biological systems of"Acid soil tolerance"in higher plants. This may lead to improve crop tolerance to acid stress factors by molecular breeding and other alternative strategies.

Research Areas


Academic & Professional Experience

Gifu University Faculty of Applied Biological Sciences
Reseuch Assistant
Associate Professor
- Associate Professor


Graduate School, Division of Agriculture, Tohoku University

Published Papers

Richa Srivastava, Sanjeev Kumar, Yasufumi Kobayashi, Kazutaka Kusunoki, Prateek Tripathi, Yuriko Kobayashi, Hiroyuki Koyama, Lingaraj Sahoo
Scientific Reports   8    Dec 2018
© 2018, The Author(s). The seminal participation of WRKY transcription factors in plant development, metabolism and in the governance of defense mechanism implicated their gaining importance for genomic and functional studies. The recent release o...
Kazutaka Kusunoki, Yasufumi Kobayashi, Yuriko Kobayashi, Hiroyuki Koyama
Soil Science and Plant Nutrition   64 469-481   Jul 2018
© 2018, © 2018 Japanese Society of Soil Science and Plant Nutrition. Under abiotic stress conditions, plants actively modify the transcriptome either for activation of stress tolerance mechanisms or adjustment of biological processes for adaptatio...
Malek Marian, Tomoki Nishioka, Hiroyuki Koyama, Haruhisa Suga, Masafumi Shimizu
Applied Soil Ecology   128 71-80   Jul 2018
© 2018 Elsevier B.V. In this study, we aimed to identify potential biocontrol agents capable of suppressing tomato bacterial wilt caused by Ralstonia pseudosolanacearum. In total, 441 bacteria were isolated from the rhizosphere soil of tomato, Chi...
Tadao Wagatsuma, Eriko Maejima, Toshihiro Watanabe, Tomonobu Toyomasu, Masaharu Kuroda, Toshiya Muranaka, Toshiya Muranaka, Kiyoshi Ohyama, Akifumi Ishikawa, Masami Usui, Shahadat Hossain Khan, Hayato Maruyama, Keitaro Tawaraya, Yuriko Kobayashi, Hiroyuki Koyama
Journal of Experimental Botany   69 567-577   Jan 2018
© The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. Aluminum-sensitive rice (Oryza sativa L.) cultivars showed increased Al tolerance under dark conditions, because less Al accumulated in t...
A. Kundu, S. Das, S. Basu, Y. Kobayashi, Y. Kobayashi, H. Koyama, M. Ganesan, M. Ganesan
Plant Biology      Jan 2018
© 2018 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands Aluminum (Al) and proton (H+) ions are major acid soil stress factors deleteriously affecting plant root growth and crop yield. In our preliminary studies,...
Abhijit Arun Daspute, Yuriko Kobayashi, Sanjib Kumar Panda, Bashasab Fakrudin, Yasufumi Kobayashi, Mutsutomo Tokizawa, Satoshi Iuchi, Arbind Kumar Choudhary, Yoshiharu Y. Yamamoto, Hiroyuki Koyama
Planta   247 201-214   Jan 2018
© 2017, Springer-Verlag GmbH Germany. Main conclusion: Al-responsive citrate-transportingCcMATE1function and its regulation byCcSTOP1were analyzed usingNtSTOP1-KD tobacco- and pigeonpea hairy roots, respectively, CcSTOP1 binding sequence ofCcMATE1...
Abhijit A. Daspute, Ayan Sadhukhan, Mutsutomo Tokizawa, Yuriko Kobayashi, Sanjib K. Panda, Sanjib K. Panda, Hiroyuki Koyama
Frontiers in Plant Science   8    Aug 2017
© 2017 Daspute, Sadhukhan, Tokizawa, Kobayashi, Panda and Koyama. Aluminum (Al) rhizotoxicity is one of the major environmental stresses that decrease global food production. Clarifying the molecular mechanisms underlying Al tolerance may contribu...
Ayan Sadhukhan, Yuriko Kobayashi, Yuki Nakano, Satoshi Iuchi, Masatomo Kobayashi, Lingaraj Sahoo, Hiroyuki Koyama
Molecular Plant   10 1082-1094   Aug 2017
© 2017 The Author Hydrogen peroxide (H2O2) is a reactive oxygen species that affects cell signaling in various plant defense responses and induces programmed cell death. To identify the new components associated with H2O2 signaling and tolerance, ...
Yoshiharu Y. Yamamoto, Yoshiharu Y. Yamamoto, Yoshiharu Y. Yamamoto, Yoshiharu Y. Yamamoto, Hiroyuki Ichida, Ayaka Hieno, Daichi Obata, Mutsutomo Tokizawa, Mika Nomoto, Yasuomi Tada, Kazutaka Kusunoki, Hiroyuki Koyama, Hiroyuki Koyama, Natsuki Hayami
DNA Research   24 271-278   Jun 2017
© The Author 2017. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. In our previous study, a methodology was established to predict transcriptional regulatory elements in promoter sequences using transcriptome data ...
Mutsutomo Tokizawa, Kazutaka Kusunoki, Hiroyuki Koyama, Hiroyuki Koyama, Atsushi Kurotani, Tetsuya Sakurai, Tetsuya Sakurai, Yutaka Suzuki, Yutaka Suzuki, Tomoaki Sakamoto, Tomoaki Sakamoto, Tetsuya Kurata, Tetsuya Kurata, Yoshiharu Y. Yamamoto, Yoshiharu Y. Yamamoto, Yoshiharu Y. Yamamoto, Yoshiharu Y. Yamamoto
Plant Journal   90 587-605   May 2017
© 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd Information about transcription start sites (TSSs) provides baseline data for the analysis of promoter architecture. In this paper we used paired- and single-end deep sequencing t...
Kazutaka Kusunoki, Yuki Nakano, Keisuke Tanaka, Yoichi Sakata, Hiroyuki Koyama, Yuriko Kobayashi
Plant Cell and Environment   40 249-263   Feb 2017
© 2016 John Wiley & Sons Ltd Differences in the expression levels of aluminium (Al) tolerance genes are a known determinant of Al tolerance among plant varieties. We combined transcriptomic analysis of six Arabidopsis thaliana accessions with co...
Umakanta Chowra, Emiko Yanase, Hiroyuki Koyama, Sanjib Kumar Panda
Protoplasma   254 293-302   Jan 2017
© 2016, Springer-Verlag Wien. Aluminium-induced oxidative damage caused by excessive ROS production was evaluated in black gram pulse crop. Black gram plants were treated with different aluminium (Al3+) concentrations (10, 50 and 100 μM with pH 4....
Katsunobu Sawaki, Yoshiharu Sawaki, Chen Ri Zhao, Yuriko Kobayashi, Hiroyuki Koyama
Plant and Soil   409 131-142   Dec 2016
© 2016, Springer International Publishing Switzerland. Background and aims: Crop production is impaired by excessive soil minerals and salts, such as sodium chloride, heavy metals, and aluminum (Al). These stressors may induce specific damage- and...
Devendra Kumar Maravi, Sanjeev Kumar, Prabin Kumar Sharma, Yasufumi Kobayashi, Yasufumi Kobayashi, Vaibhav V. Goud, Vaibhav V. Goud, Nozomu Sakurai, Hiroyuki Koyama, Lingaraj Sahoo, Lingaraj Sahoo
Biotechnology for Biofuels   9    Oct 2016
© 2016 The Author(s). Background: Jatropha curcas is an important biofuel crop due to the presence of high amount of oil in its seeds suitable for biodiesel production. Triacylglycerols (TAGs) are the most abundant form of storage oil in plants. D...
Yuriko Kobayashi, Ayan Sadhukhan, Ayan Sadhukhan, Tanveer Tazib, Yuki Nakano, Kazutaka Kusunoki, Mohamed Kamara, Mohamed Kamara, Radhouane Chaffai, Radhouane Chaffai, Satoshi Iuchi, Lingaraj Sahoo, Masatomo Kobayashi, Owen A. Hoekenga, Hiroyuki Koyama
Plant Cell and Environment   39 918-934   Apr 2016
© 2016 John Wiley & Sons Ltd. Plants have evolved a series of tolerance mechanisms to saline stress, which perturbs physiological processes throughout the plant. To identify genetic mechanisms associated with salinity tolerance, we performed lin...
Tanveer Tazib, Yuriko Kobayashi, Hiroyuki Koyama, Tsutomu Matsui
Euphytica   203 629-642   Nov 2015
© 2014, Springer Science+Business Media Dordrecht. Quantitative trait loci (QTLs) for anther length (AL), length of the dehiscence that forms at the apex of the anther [apical dehiscence length (ADL)] and at the base of the anther [basal dehiscenc...
Tadao Wagatsuma, Md Shahadat Hossain Khan, Toshihiro Watanabe, Eriko Maejima, Hitoshi Sekimoto, Takao Yokota, Takeshi Nakano, Takeshi Nakano, Takeshi Nakano, Tomonobu Toyomasu, Keitaro Tawaraya, Hiroyuki Koyama, Matsuo Uemura, Satoru Ishikawa, Takashi Ikka, Akifumi Ishikawa, Takeshi Kawamura, Satoshi Murakami, Nozomi Ueki, Asami Umetsu, Takayuki Kannari
Journal of Experimental Botany   66 907-918   Jan 2015
© The Author 2014. Several studies have shown that differences in lipid composition and in the lipid biosynthetic pathway affect the aluminium (Al) tolerance of plants, but little is known about the molecular mechanisms underlying these difference...
Mutsutomo Tokizawa, Yuriko Kobayashi, Tatsunori Saito, Masatomo Kobayashi, Satoshi Iuchi, Mika Nomoto, Yasuomi Tada, Yoshiharu Y. Yamamoto, Hiroyuki Koyama
Plant Physiology   167 991-1003   Jan 2015
© 2015 American Society of Plant Biologists. All rights reserved. In Arabidopsis (Arabidopsis thaliana) the root apex is protected from aluminum (Al) rhizotoxicity by excretion of malate, an Al chelator, by ALUMINUM-ACTIVATED MALATE TRANSPORTER1 (...
MA Jian Feng, KOBAYASHI Takanori, YAMAJI Naoki, KOBAYASHI Yasufumi, KOYAMA Hiroyuki, IWAMOTO Akitoshi, SAKURAI Gen
Japanese Journal of Soil Science and Plant Nutrition   86(3) 232-236   2015
Yuriko Kobayashi, Yoshinao Ohyama, Yasufumi Kobayashi, Hiroki Ito, Satoshi Iuchi, Miki Fujita, Cheng Ri Zhao, Tazib Tanveer, Markkandan Ganesan, Masatomo Kobayashi, Hiroyuki Koyama
Molecular Plant   7 311-322   Jan 2014
The zinc-finger protein STOP1 (sensitive to proton rhizotoxicity 1) regulates transcription of multiple genes critical for tolerance to aluminum (Al) and low pH in Arabidopsis. We evaluated the contributions of genes that are suppressed in the sto...
Mohamed M. Kamara, Mohamed M. Kamara, Ibrahim S. El-Degwy, Hiroyuki Koyama
Australian Journal of Crop Science   8 1336-1342   Jan 2014
This study was conducted to estimate general (GCA) and specific (SCA) combining ability effects of some maize inbred lines for grain yield and other agronomic traits by using line × tester analysis under two nitrogen fertilizer levels. Ten white m...
Yoshiharu Sawaki, Yuriko Kobayashi, Yuriko Kobayashi, Tomonori Kihara-Doi, Nobuyuki Nishikubo, Tetsu Kawazu, Masatomo Kobayashi, Yasufumi Kobayashi, Satoshi Iuchi, Hiroyuki Koyama, Shigeru Sato
Plant Science   223 8-15   Jan 2014
Tolerance to soil acidity is an important trait for eucalyptus clones that are introduced to commercial forestry plantations in pacific Asian countries, where acidic soil is dominant in many locations. A conserved transcription factor regulating a...
M. Alamgir Hossain, M. Alamgir Hossain, M. Ashrafuzzaman, M. Ashrafuzzaman, A. K.M.Zakir Hossain, A. K.M.Zakir Hossain, Mohd Razi Ismail, H. Koyama
The Scientific World Journal   2014    Jan 2014
Aluminum (Al) sensitive wheat cultivar kalyansona was grown for 14 d in a range of Ca solution (125, 625, and 2500 M) plus other nutrients without Al. At 14 d after Ca treatment, half of these plants were harvested (H1), and the rest of the plants...
Ayaka Hieno, Hushna Ara Naznin, Mitsuro Hyakumachi, Mitsuro Hyakumachi, Tetsuya Sakurai, Mututomo Tokizawa, Hiroyuki Koyama, Hiroyuki Koyama, Naoki Sato, Tomoaki Nishiyama, Mitsuyasu Hasebe, Mitsuyasu Hasebe, Andreas D. Zimmer, Daniel Lang, Ralf Reski, Stefan A. Rensing, Junichi Obokata, Yoshiharu Y. Yamamoto, Yoshiharu Y. Yamamoto, Yoshiharu Y. Yamamoto
Nucleic Acids Research   42    Jan 2014
ppdb (http://ppdb.agr.gifu-u.ac.jp) is a plant promoter database that provides information on transcription start sites (TSSs), core promoter structure (TATA boxes, Initiators, Y Patches, GA and CA elements) and regulatory element groups (REGs) as...
Ayan Sadhukhan, Yasufumi Kobayashi, Yuriko Kobayashi, Mutsutomo Tokizawa, Yoshiharu Y. Yamamoto, Satoshi Iuchi, Hiroyuki Koyama, Sanjib Kumar Panda, Lingaraj Sahoo
Planta   240 645-664   Jan 2014
Main conclusion: VuDREB2A exists in cowpea as a canonical DREB2-type transcription factor, having the ability to bind dehydration-responsive elements in vitro and confer enhanced drought resistance in transgenic Arabidopsis. Cowpea (Vigna unguicul...
Yasufumi Kobayashi, Yuriko Kobayashi, Toshihiro Watanabe, Jon E. Shaff, Hiroyuki Ohta, Leon V. Kochian, Tadao Wagatsuma, Thomas B. Kinraide, Hiroyuki Koyama
Plant Physiology   163 180-192   Sep 2013
Al3+ and H++ toxicities predicted to occur at moderately acidic conditions (pH+ [water] = 5-5.5) in low-Ca soils were characterized by the combined approaches of computational modeling of electrostatic interactions of ions at the root plasma membr...
Yasufumi Kobayashi, Venkatachalam Lakshmanan, Yuriko Kobayashi, Mminatsu Aasai, Satoshi Iiuchi, Mmasatomo Kobayashi, Hharsh P. Bais, Hhiroyuki Koyama
Plant Signaling and Behavior   8    Sep 2013
AtALMT1 (Arabidopsis thaliana ALuminum activated Malate Transporter 1) encodes an Arabidopsis thaliana malate transporter that has a pleiotropic role in Arabidopsis stress tolerance. Mmalate released through AtALMT1 protects the root tip from Aal ...
Yoshinao Ohyama, Hiroki Ito, Yuriko Kobayashi, Takashi Ikka, Akio Morita, Masatomo Kobayashi, Ryujiro Imaizumi, Toshio Aoki, Kenji Komatsu, Yoichi Sakata, Satoshi Iuchi, Hiroyuki Koyama
Plant Physiology   162 1937-1946   Aug 2013
Aluminum (Al) and proton (H+) tolerances are essential traits for plants to adapt to acid soil environments. In Arabidopsis (Arabidopsis thaliana), these tolerances are mediated by a zinc-finger transcription factor, SENSITIVE TO PROTON RHIZOTOXIC...
Ayaka Hieno, Hushna Ara Naznin, Katsunobu Sawaki, Hiroyuki Koyama, Hiroyuki Koyama, Yusaku Sakai, Haruka Ishino, Mitsuro Hyakumachi, Mitsuro Hyakumachi, Yoshiharu Y. Yamamoto, Yoshiharu Y. Yamamoto
Methods in Enzymology   527 221-237   Jul 2013
Hydrogen peroxide acts as a signaling molecule mediating the acquisition of tolerance to both biotic and abiotic stresses. Identification of marker genes for H2O2 response could help to intercept the signaling network of stress response of plants....
Yasufumi Kobayashi, Yuriko Kobayashi, Miki Sugimoto, Venkatachalam Lakshmanan, Satoshi Iuchi, Masatomo Kobayashi, Harsh P. Bais, Hiroyuki Koyama
Plant Physiology   162 732-740   Jun 2013
In Arabidopsis (Arabidopsis thaliana), malate released into the rhizosphere has various roles, such as detoxifying rhizotoxic aluminum (Al) and recruiting beneficial rhizobacteria that induce plant immunity. ALUMINUM-ACTIVATED MALATE TRANSPORTER1 ...
Yoshiharu Sawaki, Tomonori Kihara-Doi, Yuriko Kobayashi, Nobuyuki Nishikubo, Tetsu Kawazu, Yasufumi Kobayashi, Hiroyuki Koyama, Shigeru Sato
Planta   237 979-989   Jan 2013
Many plant species excrete organic acids into the rhizosphere in response to aluminum stress to protect sensitive cells from aluminum rhizotoxicity. When the roots of Eucalyptus camaldulensis, a major source of pulp production, were incubated in a...
Japanese Journal of Soil Science and Plant Nutrition   84(2) 136-141   2013
無菌生物 = Japanese journal of germfree life and gnotobiology   42(2) 69-72   Dec 2012
IKKA Takashi, MORITA Akio, KOYAMA Hiroyuki
Japanese Journal of Soil Science and Plant Nutrition   83(4) 449-454   2012
Radhouane Chaffai, Radhouane Chaffai, Hiroyuki Koyama
Advances in Botanical Research   60 1-49   Nov 2011
Understanding of molecular mechanisms of heavy metal (HM) tolerance is an important issue in plant biology because HM contamination has become a potential risk for modern agriculture. HMs generally cause toxicity to plants at very low amounts, whi...
M. A. Hossain, Mohd Razi Ismail, M. Ashrafuzzaman, H. Koyama
Australian Journal of Crop Science   5 1157-1162   Sep 2011
Aluminum stress inhibited root growth and caused serious oxidative damage in wheat (Triticum aestivum L.) seedlings. Under Al stress, added Ca significantly improved root growth, decreased Al, H 2O 2 and TBARS content and increased O 2- generation...
Yoshiharu Y. Yamamoto, Yohei Yoshioka, Mitsuro Hyakumachi, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Mutsutomo Tokizawa, Hiroyuki Koyama
BMC Plant Biology   11    Feb 2011
Background: Phytohormones organize plant development and environmental adaptation through cell-to-cell signal transduction, and their action involves transcriptional activation. Recent international efforts to establish and maintain public databas...
無菌生物 = Japanese journal of germfree life and gnotobiology   40(2)    Dec 2010
Tatsunori Masatani, Naoto Ito, Naoto Ito, Kenta Shimizu, Yuki Ito, Keisuke Nakagawa, Yoshiharu Sawaki, Hiroyuki Koyama, Makoto Sugiyama, Makoto Sugiyama
Journal of Virology   84 4002-4012   Apr 2010
The rabies virus Ni-CE strain causes nonlethal infection in adult mice after intracerebral inoculation, whereas the parental Nishigahara (Ni) strain kills mice. We previously reported that the chimeric CE(NiN) strain with the N gene from the Ni st...
Cheng Ri Zhao, Yoshiharu Sawaki, Nozomu Sakurai, Daisuke Shibata, Hiroyuki Koyama
Soil Science and Plant Nutrition   56 150-162   Feb 2010
Alteration of metabolic processes is a common adaptive response of plants to various stress conditions and is likely to be under complex regulatory control. To understand the metabolic responses to rhizotoxic treatments in Arabidopsis thaliana, tr...
Makino Amane, YAMAYA Tomoyuki, KAMATA Atsushi, OCHIAI Kumiko, KOYAMA Hiroyuki, MA Jian Feng, WATANABE Toshihiro
Japanese Journal of Soil Science and Plant Nutrition   81(2) 181-189   2010
Journal of germfree life and gnotobiology   39(2) 57-60   Dec 2009
Tanveer Tazib, Yuriko Kobayashi, Takashi Ikka, Cheng Ri Zhao, Satoshi Iuchi, Masatomo Kobayashi, Kazuhiko Kimura, Hiroyuki Koyama
Physiologia Plantarum   137 235-248   Nov 2009
Association mapping analysis of Cd, Cu and H 2O 2 tolerance, judged by relative root length (RRL: % of root length in stress condition relative to that in control condition), and Cd and Cu translocation ratios (amount of metal in the shoot to the ...
無菌生物 = Japanese journal of germfree life and gnotobiology   39(1)    Sep 2009
Tanveer Tazib, Takashi Ikka, Keishi Kuroda, Yuriko Kobayashi, Kazuhiko Kimura, Hiroyuki Koyama
Physiologia Plantarum   136 395-406   Aug 2009
To understand mechanisms of cadmium (Cd) tolerance variation associated with root elongation in Arabidopsis thaliana, quantitative trait loci (QTLs) and epistasis were analyzed using relative root length (RRL: % of the root length in +Cd to -Cd) a...
Yoshiharu Sawaki, Satoshi Iuchi, Yasufumi Kobayashi, Yuriko Kobayashi, Takashi Ikka, Nozomu Sakurai, Miki Fujita, Kazuo Shinozaki, Daisuke Shibata, Masatomo Kobayashi, Hiroyuki Koyama
Plant Physiology   150 281-294   May 2009
The Arabidopsis (Arabidopsis thaliana) mutant stop1 (for sensitive to proton rhizotoxicity1) carries a missense mutation at an essential domain of the histidine-2-cysteine-2 zinc finger protein STOP1. Transcriptome analyses revealed that various g...
Cheng Ri Zhao, Takashi Ikka, Yoshiharu Sawaki, Yuriko Kobayashi, Yuji Suzuki, Takashi Hibino, Shigeru Sato, Nozomu Sakurai, Daisuke Shibata, Hiroyuki Koyama
BMC Plant Biology   9    Apr 2009
Background: Rhizotoxic ions in problem soils inhibit nutrient and water acquisition by roots, which in turn leads to reduced crop yields. Previous studies on the effects of rhizotoxic ions on root growth and physiological functions suggested that ...
M. Shahadat Hossain Khan, Keitarou Tawaraya, Hiroshi Sekimoto, Hiroyuki Koyama, Yuriko Kobayashi, Tetsuya Murayama, Masaru Chuba, Mihoko Kambayashi, Yoshihito Shiono, Matsuo Uemura, Satoru Ishikawa, Tadao Wagatsuma
Physiologia Plantarum   135 73-83   Jan 2009
We investigated variations in aluminum (Al) tolerance among rice plants, using ancestor cultivars from the family line of the Al-tolerant and widely cultivated Japonica cultivar, Sasanishiki. The cultivar Rikuu-20 was Al sensitive, whereas a close...
Yuriko Kobayashi, Yuriko Kobayashi, Keishi Kuroda, Keisuke Kimura, Jennafer L. Southron-Francis, Aya Furuzawa, Kazuhiko Kimura, Satoshi Iuchi, Masatomo Kobayashi, Gregory J. Taylor, Hiroyuki Koyama
Plant Physiology   148 969-980   Oct 2008
Copper (Cu) is an essential element in plant nutrition, but it inhibits the growth of roots at low concentrations. Accessions of Arabidopsis (Arabidopsis thaliana) vary in their tolerance to Cu. To understand the molecular mechanism of Cu toleranc...
Takashi Ikka, Yuriko Kobayashi, Tanveer Tazib, Hiroyuki Koyama
Plant Science   175 533-538   Oct 2008
Aluminum (Al) tolerance is one of the most important target traits for improving crop production in acid soil. To understand the complex molecular mechanism of Al tolerance, QTL analysis in Arabidopsis would be one useful approach. Using relative ...
Md Motaher Hossain, Farjana Sultana, Mayumi Kubota, Hiroyuki Koyama, Mitsuro Hyakumachi
Journal of General Plant Pathology   74 213-221   Jun 2008
The culture filtrate (CF) from the plant growth-promoting fungus Phoma sp. GS8-1 was found to induce systemic resistance in Arabidopsis thaliana against the bacterial leaf speck pathogen Pseudomonas syringae pv. tomato DC3000 (Pst), and the underl...
Satoshi Iuchi, Satoshi Iuchi, Yuriko Kobayashi, Hiroyuki Koyama, Masatomo Kobayashi
Plant Signaling and Behavior   3 128-130   Jan 2008
Under acid soil condition, rhizotoxic ions such as aluminum and protons cause severe yield loss in food and biomass production via inhibition of root growth and/or enhancement of sensitivity to drought stress. Therefore, improvement of crop tolera...
KOYAMA Hiroyuki, KOBAYASHI Yasufumi, Kinraide T. B., WAGATSUMA Tadao
Japanese Journal of Soil Science and Plant Nutrition   79(5) 500-504   2008
Journal of germfree life and gnotobiology   37(2) 111-113   Dec 2007
Md Motaher Hossain, Farjana Sultana, Mayumi Kubota, Hiroyuki Koyama, Mitsuro Hyakumachi
Plant and Cell Physiology   48 1724-1736   Dec 2007
Arabidopsis thaliana grown in soil amended with barley grain inocula of Penicillium simplicissimum GP17-2 or receiving root treatment with its culture filtrate (CF) exhibited clear resistance to Pseudomonas syringae pv. tomato DC3000 (Pst). To ass...
Yuriko Kobayashi, Takashi Ikka, Kazuhiko Kimura, Orito Yasuda, Hiroyuki Koyama
Functional Plant Biology   34 984-994   Nov 2007
The mechanism of lanthanum (La3+) toxicity on root growth of Arabidopsis was studied by physiological and genetic approaches using Landsberg erecta (Ler) × Columbia (Col) recombinant inbred lines (RILs) and other natural accessions. Quantitative t...
Yuriko Kobayashi, Yuriko Kobayashi, Owen A. Hoekenga, Hirotaka Itoh, Midori Nakashima, Shoichiro Saito, Jon E. Shaff, Lyza G. Maron, Miguel A. Piñeros, Leon V. Kochian, Hiroyuki Koyama
Plant Physiology   145 843-852   Nov 2007
Malate transporters play a critical role in aluminum (Al) tolerance responses for some plant species, such as Arabidopsis (Arabidopsis thaliana). Here, we further characterize AtALMT1, an Arabidopsis aluminum-activated malate transporter, to clari...
Takashi Ikka, Yuriko Kobayashi, Satoshi Iuchi, Nozomu Sakurai, Daisuke Shibata, Masatomo Kobayashi, Hiroyuki Koyama
Theoretical and Applied Genetics   115 709-719   Sep 2007
Root growth of Arabidopsis thaliana is inhibited by proton rhizotoxicity in low ionic strength media when the pH of the medium is lower than 5.0. QTL analysis at pH 4.7 revealed that two major QTLs on chromosome 2 and 5 and an additional six epist...
Satoshi Iuchi, Hiroyuki Koyama, Atsuko Iuchi, Yasufumi Kobayashi, Sadako Kitabayashi, Yuriko Kobayashi, Takashi Ikka, Takashi Hirayama, Takashi Hirayama, Kazuo Shinozaki, Masatomo Kobayashi
Proceedings of the National Academy of Sciences of the United States of America   104 9900-9905   Jun 2007
Acid soil syndrome causes severe yield losses in various crop plants because of the rhizotoxicities of ions, such as aluminum (Al3+). Although protons (H+) could be also major rhizotoxicants in some soil types, molecular mechanisms of their tolera...
Journal of germfree life and gnotobiology   36(1) 46-49   Sep 2006
Tomonori Kihara, Cheng R. Zhao, Yuriko Kobayashi, Eiji Takita, Tetsu Kawazu, Hiroyuki Koyama
Bioscience, Biotechnology and Biochemistry   70 1780-1783   Aug 2006
Transgenic Arabidopsis thaliana plants carrying a single copy of integrated DNA can be identified by single-step genomic polymerase chain reaction. The reaction employs two sets of primer pairs with the same melting temperature that amplify the am...
Owen A. Hoekenga, Owen A. Hoekenga, Lyza G. Maron, Lyza G. Maron, Miguel A. Piñeros, Miguel A. Piñeros, Geraldo M.A. Cançado, Geraldo M.A. Cançado, Jon Shaff, Jon Shaff, Yuriko Kobayashi, Peter R. Ryan, Bei Dong, Emmanuel Delhaize, Takayuki Sasaki, Hideaki Matsumoto, Yoko Yamamoto, Hiroyuki Koyama, Leon V. Kochian
Proceedings of the National Academy of Sciences of the United States of America   103 9738-9743   Jun 2006
Aluminum (Al) tolerance in Arabidopsis is a genetically complex trait, yet it is mediated by a single physiological mechanism based on Al-activated root malate efflux. We investigated a possible molecular determinant for Al tolerance involving a h...
A. Hossain, M. Hossain, M. Asgar, T. Tosaki, H. Koyama, T. Hara, T. Hara
Journal of Plant Nutrition   29 601-613   Apr 2006
The present study was conducted to investigate the cell-wall polysaccharides and hydroxycinnamates in wheat plants (Triticum aestivum L.) under aluminum (Al) stress at a higher level of calcium (Ca) supply. Seedlings were grown in nutrient solutio...
A. K.M. Zakir Hossain, Hiroyuki Koyama, Tetsuo Hara
Journal of Plant Physiology   163 39-47   Jan 2006
The present study was conducted to investigate the cell wall properties in two wheat (Triticum aestivum L.) cultivars differing in their sensitivity to Al stress. Seedlings of Al-resistant, Inia66 and Al-sensitive, Kalyansona cultivars were grown ...
Journal of germfree life and gnotobiology   35(2) 146-148   Dec 2005
Yuriko Kobayashi, Yachiyo Furuta, Takashi Ohno, Tetsuo Hara, Hiroyuki Koyama
Plant, Cell and Environment   28 1516-1524   Dec 2005
Quantitative trait loci (QTL) analysis of aluminium (Al) tolerance was performed using Ler/Cvi recombinant inbred (RI) lines of Arabidopsis thaliana. Relative root length (RRL) (root length with 4 μm Al/root length with no Al at pH 5.0) on day 5 w...
A. K.M.Zakir Hossain, Takashi Ohno, Hiroyuki Koyama, Tetsuo Hara
Plant and Soil   276 193-204   Oct 2005
The present study was conducted to investigate the effects of enhanced Ca supply on Al toxicity in relation to cell wall properties in two wheat (Triticum aestivum L.) cultivars differing in Al resistance. Seedlings of Al-tolerant Inia66 and Al-se...
M. Alamgir Hossain, A. K.M.Zakir Hossain, Tomonori Kihara, Hiroyuki Koyama, Tetsuo Hara
Soil Science and Plant Nutrition   51 223-230   Apr 2005
Wheat (Triticum aestivum cv. Kalyansona) seedlings were exposed to 0, 10, 50, and 100 μM Al in a 250 μM CaCl2solution for 24 h at pH 4.5. Root elongation decreased gradually, while the Al content markedly increased with increasing supply of Al in ...
A. K.M.Zakir Hossain, Md Ali Asgar, M. Alamgir Hossain, Toshihiko Tosaki, Hiroyuki Koyama, Tetsuo Hara
Soil Science and Plant Nutrition   51 43-49   Feb 2005
The effects of B and Ca treatments on root growth, nutrient localization and cell wall properties in wheat (Triticum aestivum L.) plants with and without Al stress were investigated. Seedlings were grown hydroponically in a complete nutrient solut...
Koyama Hiroyuki
Japanese Journal of Soil Science and Plant Nutrition   76(3) 331-334   2005
Journal of germfree life and gnotobiology   34(2) 87-91   Dec 2004
KOYAMA Hiroyuki, KAWAZU Tetsu
化学と生物   42(10) 651-657   Oct 2004
Yuji Suzuki, Yuji Suzuki, Tetsu Kawazu, Hiroyuki Koyama, Hiroyuki Koyama
BioTechniques   37 542-544   Oct 2004
Takashi Ohno, Shinobu Nakahira, Yuji Suzuki, Tomomi Kani, Tetsuo Hara, Hiroyuki Koyama
Physiologia Plantarum   122 265-274   Oct 2004
The citrate efflux of a carrot mutant cell line (IPG; insoluble phosphate grower) with enhanced citrate excretion is concomitant with an electrically equivalent H+ release. Furthermore, plasma membrane H +-ATPase activity (EC in the IPG ...
A. K.M.Zakir Hossain, M. Alamgir Hossain, Hiroyuki Koyama, Tetsuo Hara
Soil Science and Plant Nutrition   50 189-195   Feb 2004
Aluminum toxicity and boron deficiency are the major factors that limit plant growth and development in acid soils and in B-deficient soils. Root growth inhibition is an early symptom of AI toxicity and B deficiency. Effects of AI and B supply and...
M. Alamgir Hossain, Keisuke Ban, A. K.M.Zakir Hossain, Hiroyuki Koyama, Tetsuo Hara
Soil Science and Plant Nutrition   50 283-286   Feb 2004
A Bangladeshi wheat (Triticum aestivum) cultivar, Kalyansona, was exposed to 100 J.µ Al with varying concentrations of Ca (250, 500, 2,500 J.µM) and Mg (103, 1,029, 4,115 J.µ) in the nutrient solution for 14 d. Exposure to Al along with low concen...
Koyama Hiroyuki, Matoh Toru
Japanese Journal of Soil Science and Plant Nutrition   75(3) 381-386   2004
Tomonori Kihara, Tatsumi Wada, Yuji Suzuki, Tetsuo Hara, Hiroyuki Koyama
Plant and Cell Physiology   44 901-908   Sep 2003
Organic acid excretion plays a key role in the superior Pi-acquisition of barely soluble inorganic P sources from soils. Seedlings of white lupin (Lupinus albus L.) grown for 37 d in -P nutrient solution showed typical -P symptoms, such as low P c...
Y. Suzuki, Y. Suzuki, Y. Suzuki, T. Hibino, T. Kawazu, T. Wada, T. Kihara, H. Koyama, H. Koyama
BioTechniques   34 988-993   May 2003
Rapid extraction of total RNA from Eucalyptus leaves is difficult due to the high content of polyphenolics and polysaccharides. A rapid and simple method was developed by using an extraction buffer containing sodium isoascorbate at a concentration...
Takashi Ohno, Hiroyuki Koyama, Tetsuo Hara
Plant and Cell Physiology   44 156-162   Feb 2003
The superior ability of citrate excretion in a carrot (Daucus carota L.) mutant cell line, namely IPG (insoluble phosphate grower) [Takita et al. (1999a) Plant Cell Physiol. 40: 489] cells has been characterized in terms of citrate transport at th...
Tomonori Kihara, Takashi Ohno, Hiroyuki Koyama, Takuya Sawafuji, Tetsuo Hara
Plant and Soil   248 145-153   Jan 2003
Citrate in the cytosol is converted to isocitrate, by the action of aconitase, and then isocitrate is converted to 2-oxoglutarate by the action of NADP-specific isocitrate dehydrogenase (NADP-ICDH). This pathway is modified in a mutant carrot (Dau...
Yuriko Kobayashi, Hiroyuki Koyama
Plant and Cell Physiology   43 1526-1533   Dec 2002
Quantitative trait loci (QTLs) and epistasis for Arabidopsis thaliana aluminum (Al) tolerance were analyzed using a recombinant inbred (RI) population of 100 lines derived from a cross between Landsberg erecta and Columbia (Col). Root growth of th...
Munechika Honda, Yasunori Muramoto, Tsuyoshi Kuzuguchi, Sadanori Sawano, Masayuki Machida, Hiroyuki Koyama
Journal of Experimental Botany   53 1515-1520   Jan 2002
A simple and rapid method is described for determining the integrated T-DNA copy number and the genotype in transgenic Arabidopsis thaliana by two-step competitive PCR. First, the amount of genomic DNA in the extracts, obtained from an individual ...
KIHARA Tomonori, KOYAMA Hiroyuki, MIZUNO Hideto, HARA Tetsuo
Journal of germfree life and gnotobiology.   31(2) 45-49   Dec 2001
H. Koyama, T. Toda, T. Hara
Journal of Experimental Botany   52 361-368   May 2001
The viability of Arabidopsis thaliana (strain Landsberg) roots exposed to a low pH (4.5 or 4.7) solution that contained 100 μM CaCl2was examined by staining with fluorescein diacetate-propidium iodide. The elongation zone of growing roots lost via...
Hiroyuki Koyama, Ayako Kawamura, Tomonori Kihara, Tetsuo Hara, Eiji Takita, Daisuke Shibata
Plant and Cell Physiology   41 1030-1037   Jan 2000
The gene for mitochondrial citrate synthase (CS) was isolated from Daucus carota (DcCS) and introduced into Arabidopsis thaliana (strain WS) using Agrobacterium tumefaciens-mediated transformation. Characteristics of citrate excretion were compare...
Eiji Takita, Hiroyuki Koyama, Tetsuo Hara
Plant and Cell Physiology   40 489-495   Jan 1999
Carbohydrate metabolism in Al-phosphate utilizing cells of carrot [designated as IPG, Koyama et al. (1992) Plant Cell Physiol. 33: 171], which grow normally in Al-phosphate medium accompanied by citrate excretion, was investigated. The excretion o...
Md Taiabur Rahman, Hiroyuki Koyama, Tetsuo Hara
Soil Science and Plant Nutrition   45 693-700   Jan 1999
Cells of two indica rice (Oryza sativa L.) varieties, one aluminum-tolerant (IAC3), and the other aluminum-sensitive (IR45), were grown in suspension culture in a medium with or without Al (0-800 µM), Si (0-2,000 µM), or Al and Si in combination a...
Eiji Takita, Hiroyuki Koyama, Yumiko Shirano, Daisuke Shibata, Tetsuo Hara
Soil Science and Plant Nutrition   45 197-205   Jan 1999
We characterized the mitochondrial citrate synthase (mtCS) in selected carrot cells designated as IPG cells (Koyama et al. 1992), which showed a mtCS activity about 1.5 times higher than that of the wild-type (WT) cells. Since the CS in both cell ...
Tetsuo Hara, Ming Hua Gu, Hiroyuki Koyama
Soil Science and Plant Nutrition   45 929-936   Jan 1999
Rice (cv: Koshihikari) plants were subjected to water-culture during 3 or 4 d with Al (0.2 and 0.8 mM) and low molecular Si (3.0 mM) or high molecular Si (3.0 mM low molecular Si+21.7 mM high molecular Si) in a 0.5 mM Ca(NO3)2solution in order to ...
Tomomi Toda, Hiroyuki Koyama, Tetsuo Hara
Bioscience, Biotechnology and Biochemistry   63 210-212   Jan 1999
In the studies of nutritional absorption and metal toxicity in the root, it is important to grow plants without technical damage. We established a simple hydroponic culture system for Arabidopsis thaliana to obtain a healthy plant having a well-de...
Tomomi Toda, Hiroyuki Koyama, Teruyo Hori, Tetsuo Hara
Soil Science and Plant Nutrition   45 419-425   Jan 1999
We compared the aluminum (A1) tolerance of three Arabidopsis thaliana ecotypes [Columbia (Col-O), Wassilewskija (Ws), and Landsberg (Ler)] and an AI-tolerant line (A1-t) selected from Ler under both hydroponic and soil culture conditions. For the ...
Hiroyuki Koyama, Eiji Takita, Ayako Kawamura, Tetsuo Hara, Daisuke Shibata
Plant and Cell Physiology   40 482-488   Jan 1999
A mitochondrial citrate synthase (CS) of Arabidopsis thaliana was introduced into carrot (Daucus carota L. cv. MS Yonsun) cells by Agrobacterium tumefaciens-mediated transformation. Transgenic cell lines had high CS activity, the highest value obs...
Gu Ming-Hua, Koyama Hiroyuki, Hara Tetuo
Japanese Journal of Soil Science and Plant Nutrition   70(6) 731-738   1999
In order to evaluate the effects of Si supply on the amelioration of Al injury of rice plants (Koshihikari), plants were water-cultured for 3 d with Al (0.8 mmol L^<-1>) and low molecular Si (3.0 mmol L^<-1>) or high molecular Si (3.0 mmol L^<-1> ...
Md Taiabur Rahman, Kazunobu Kawamura, Hiroyuki Koyama, Tetsuo Hara
Soil Science and Plant Nutrition   44 423-431   Jan 1998
Effects of Al (0–100 μM) and Si (0–2,000 μM) supplied singly or in combination on root growth of different rice varieties were examined under hydroponic conditions. Al addition inhibited root elongation of rice plants, and the inhibition increased...
Gu Ming-Hua, Koyama Hiroyuki, Hara Tetsuo
Japanese Journal of Soil Science and Plant Nutrition   69(5) 498-505   1998
Rice plants were water-cultured with a combination of aluminum (0, 0.2, 0.8 mmol L^<-1>) and silicon (0, 3.0 mmol L^<-1>) in a 0.5 mmol L^<-1> Ca(NO_3)_2 solution, and both elements with different chemical forms in the plant organs were determined...
TAKITA Eiji, KOYAMA Hiroyuki, HARA Tetsuo, OJIMA Kunihiko
Journal of germfree life and gnotobiology.   27(2) 80-83   Dec 1997
Munechika Honda, Hiroyuki Koyama, Tetsuo Hara
Soil Science and Plant Nutrition   42 401-406   Jan 1996
Aluminum toxicity and related nutrient deficiencies, such as phosphorus deficiency, are considered to be major environmental constraints on food production world wide (Foy 1983). Toxic effects of Al in cells are characterized by the inhibition of ...
H. Koyama, K. Ojima, T. Yamaya, Y. Sonoda
Plant and Soil   171 179-183   Apr 1995
Plantlets of carrot (Daucus carota L.) were regenerated from two types of cell lines. One type was selected as ionic-Al tolerant (IAT) cells, while the second type featured Al-phosphate utilizing cells (IPG). Their tolerance characteristics were i...
Hiroyuki Koyama, Hiroyuki Koyama, Tomomi Toda, Hiromi Kojima, Tetsuo Hara
Soil Science and Plant Nutrition   41 173-176   Mar 1995
Arabidopsis thaliana displays useful characteristics for studies on plant molecular biology, such as small genome size and the suitability for either tissue culture system or transformation (Schmidt and Willmitizer 1991). In addition, because a re...


Cheng Ri Zhao, Yoshiharu Y. Yamamoto, Hiroyuki Koyama
Omics and Plant Abiotic Stress Tolerance   128-132   Dec 2011
Rhizotoxic ions inhibit root growth, resulting in reduced yield of various crop plants. It is thus improvement of tolerance of roots to rhizotoxic ions, one of the most important targets in plant breeding, to improve the productivity of crops in v...
Re-evaluation of a carrot cell line previously selected as aluminum-tolerant cells.(共著)
Physiol. Plant.   74 683-687   1988
Characterization of two obacco cell lines selected to grow in the presence of either ionic Al or Al-phosphate.(共著)
Soil Sci. Plant Nutr.   35 545-551   1989
Utilization of anhydrous aluminium phosphate as a sole source of phosphate by a selected carrot cell line.(共著)
Plant Cell Physiol.   31 173-177   1990
Aluminum-tolerance of Carrot(Daucus carota. L.)plants regenerated from selected cell cultures.(共著)
Soil Sci. Plant Nutr.   37 699-705   1991

Books etc

Feb 2005   
Apr 1994   
Jun 2002   
Aug 2002   

Research Grants & Projects

Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(A))
Project Year: 2010 - 2013    Investigator(s): HIROYUKI KOYAMA
STOP1, a zinc finger protein, regulates transcription of Al and proton tolerance genes in Arabidopsis. We characcterized function of orthologous genes in various plant species, including woody plants, grass plants, and bryophyte. All plants conser...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 2007 - 2009    Investigator(s): Hiroyuki KOYAMA
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 2003 - 2006    Investigator(s): Hiroyuki KOYAMA
Development of P-efficient plants, which can grow better than regular crop plants by enhanced ability of P-uptake from soil, would be important targets in plant breeding. Enhancement of organic acid excretion from the root, which seemed to be invo...
Molecular Breeding in Acid soil Tolerant Plants
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research
Project Year: 2010 - 2013