Molecular and cellular pathogenesis of herpesviruses／My interest is pathogenicity of herpesvirus, especially equine herpesvirus type 1, and immune response of the host at molecular and cellular point of view. The purpose of this research is to reveal the mechanism of virus infection and toestablish the prevention and control of the diseases.／Investigation of potential emerging and reemerging pathogens in wild animals／This research has bee examined in order to know the current situation of bacteria, viruses, and chlamydia in wild animals and to predict the future occurrence of emerging diseases.／The biology of Chlamydophila (Chlamydia) psittaci／I have characterized a zoonotic agent, Chlamydophila psittaci, at a molecular point of view. The results has been applied to epidemiological investigation and control measures of psittacosis.
Journal of Veterinary Medical Science 77(7) 777-782 Jan 2015 [Refereed]
? 2015 The Japanese Society of Veterinary Science. Chlamydia pecorum (designated 22-58) was isolated in 2010 in HmLu-1 cells from the jejunum of a calf which died of necrotizing enterocolitis in Yamaguchi Prefecture, Japan. Immunohistochemical sta...
Journal of Veterinary Medical Science 76(9) 1309-1312 Jan 2014 [Refereed]
? 2014 The Japanese Society of Veterinary Science. A strain of equine herpesvirus type 1 (EHV-1) was isolated from zebra. This strain, called “zebra-borne EHV-1”, was also isolated from an onager and a gazelle in zoological gardens in U.S.A. The f...
? 2014 Elsevier B.V. Intracellular localization of Equine herpesvirus type 1 (EHV-1) tegument protein VP22 was examined by using a plasmid that expressed VP22 fused with an enhanced green fluorescent protein (EGFP). Also a recombinant EHV-1 expres...
Research bulletin of the Faculty College of Agriculture Gifu University 50 259-263
Chlamydial complement fixing (CF) antibodies were investigated for 1,048 cow and 544 pig sera from Aichi and Gifu Prefectures, and 1,103 horse sera from Ibaragi, Gifu and Tokyo Prefectures from 1980 to 1983 in Japan. An average positive rate of co...
Research bulletin of the Faculty College of Agriculture Gifu University 51 201-205
An outbreak of avian pox occurred in psittacine birds, parrots, lovebirds and cockatiels in a wholesaler. Clinical signs were blepharitis, conjunctivitis, keratitis and ophthalmitis. Lesions included periocular ulcerations and scabs. Histologicall...
Research bulletin of the Faculty College of Agriculture Gifu University 50 271-277
An indirect hemagglutination test for detecting avian chlamydial antibodies was established. Sensitized red blood cells were prepared using sodium N-laurylsarcosinate soluble fraction of purified elementary bodies and glutaraldehyde prefixed sheep...
Research bulletin of the Faculty College of Agriculture Gifu University 50 265-270
A system of an enzyme-linked immunosorbent assay (ELISA) was established to detect chlamydial antibodies in various animals. Purified elementary bodies (EB) which were treated with 1% Triton X-100 at a protein concentration of 1 mg/ml were diluted...
Japanese Journal of Zoo and Wildlife Medicine 12(1) 77-80
A female Japanese rock ptarmigan (Lagopus mutus japonicus) in captivity died of an unknown reason in 1985. The carcass was stored at -15℃ until 2005 and then examined to define the cause of death. Finding a nodular lesion on the cere, avian poxvir...
Japanese Journal of Zoo and Wildlife Medicine 3(1) 1-7
The prevalence of infectious agents, drug resistant-Escherichia coli and residual organochlorine in 14 species of mammals and 20 species of birds was investigated during the period 1991 to 1993. Zoonotic organisms, Yersinia enterocolitica, Y.pseud...
Research bulletin of the Faculty College of Agriculture Gifu University 49 201-207
We encountered an outbreak of non-01 vibriosis caused by Vibrio cholerae serovar 64 in pet Japanese nightingale (Leiothrix Lutea) originating from China. Isolation of non-01 Vibrio cholerae has been reported among domestic and feral ducks. No pape...
Research bulletin of the Faculty College of Agriculture Gifu University 50 251-257
Salmonella infection has been reported among several species of birds including domestic and feral types in Japan. However, little information is available concerning the occurrence and serotype of Salmonella infection in pet birds. During the 2 y...
Research bulletin of the Faculty College of Agriculture Gifu University 49 209-216
Isolation of Escherichia coli has been reported among several species of birds including domestic and feral birds. However, little information is available concerning the occurrence and serotype of E. coli infection in pet birds. During the 2 year...
Genetic information is transcribed from DNA to RNA as mRNA. And the RNA called anti-sense RNA is transcribed from the antisense strand of DNA. The purpose of this study was to clarify the function of the anti-sense RNA found in equine herpesvirus ...
Chlamydia psittaci, an obligate intracellular bacterium, is an etiological agent for human psittacosis. In this project, regarding chlamydial pathogenesis and diverse host range, we have focused on the role of the polymorphic membrane protein (pmp...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(A))
Project Year: 2009 - 2011 Investigator(s): Jun YASUDA
To monitor the environmental conservation of wildlife, we established the method of evaluation using the living body information of small antelopes in Africa. We investigated genetic characteristics and antimicrobial resistance of enteric bacteria...
The object of this research is to reveal the molecular mechanism of herpesvirus lethal encephalitis in non-natural host. Equine herpesviruses(EHVs) 1 and 9 were used. Virus genes involving in the lethal encephalitis in non-natural host were identi...
In the present study, to understand the molecular mechanism for the virulence or pathogenicity of the virulent infectious bursal disease virus (IBDV), several experiments were employed as listed below.1. Usefulness of chicken B lymphoma LSCC-DT40 ...
