論文

査読有り 筆頭著者 責任著者
2017年11月

Canine salivary glands: analysis of Rab and SNARE protein expression and SNARE complex formation with diverse tissue properties

Journal of Histochemistry & Cytochemistry
  • Hiroshi Gomi
  • ,
  • Hiromi Osawa
  • ,
  • Rie Uno
  • ,
  • Tadashi Yasui
  • ,
  • Masahiro Hosaka
  • ,
  • Seiji Torii
  • ,
  • Azuma Tsukise

65
11
開始ページ
637
終了ページ
653
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1369/0022155417732527
出版者・発行元
SAGE PUBLICATIONS LTD

The comparative structure and expression of salivary components and vesicular transport proteins in the canine major salivary glands were investigated. Histochemical analysis revealed that the morphology of the five major salivary glandsparotid, submandibular, polystomatic sublingual, monostomatic sublingual, and zygomatic glandswas greatly diverse. Immunoblot analysis revealed that expression levels of alpha-amylase and antimicrobial proteins, such as lysozyme, lactoperoxidase, and lactoferrin, differed among the different glands. Similarly, Rab proteins (Rab3d, Rab11a, Rab11b, Rab27a, and Rab27b) and soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins VAMP4, VAMP8, syntaxin-2, syntaxin-3, syntaxin-4, and syntaxin-6 were expressed at various levels in individual glands. mmunohistochemistry of Rab3d, Rab11b, Rab27b, VAMP4, VAMP8, syntaxin-4, and syntaxin-6 revealed their predominant expression in serous acinar cells, demilunes, and ductal cells. The VAMP4/syntaxin-6 SNARE complex, which is thought to be involved in the maturation of secretory granules in the Golgi field, was found more predominantly in the monostomatic sublingual gland than in the parotid gland. These results suggest that protein expression profiles in canine salivary glands differ among individual glands and reflect the properties of their specialized functions.

リンク情報
DOI
https://doi.org/10.1369/0022155417732527
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000413679600002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1369/0022155417732527
  • ISSN : 0022-1554
  • eISSN : 1551-5044
  • Web of Science ID : WOS:000413679600002

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