論文

査読有り
2013年2月

Morphology-Based Prediction of Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells

PLOS ONE
  • Fumiko Matsuoka
  • ,
  • Ichiro Takeuchi
  • ,
  • Hideki Agata
  • ,
  • Hideaki Kagami
  • ,
  • Hirofumi Shiono
  • ,
  • Yasujiro Kiyota
  • ,
  • Hiroyuki Honda
  • ,
  • Ryuji Kato

8
2
開始ページ
e55082
終了ページ
e55082
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0055082
出版者・発行元
PUBLIC LIBRARY SCIENCE

Human bone marrow mesenchymal stem cells (hBMSCs) are widely used cell source for clinical bone regeneration. Achieving the greatest therapeutic effect is dependent on the osteogenic differentiation potential of the stem cells to be implanted. However, there are still no practical methods to characterize such potential non-invasively or previously. Monitoring cellular morphology is a practical and non-invasive approach for evaluating osteogenic potential. Unfortunately, such image-based approaches had been historically qualitative and requiring experienced interpretation. By combining the non-invasive attributes of microscopy with the latest technology allowing higher throughput and quantitative imaging metrics, we studied the applicability of morphometric features to quantitatively predict cellular osteogenic potential. We applied computational machine learning, combining cell morphology features with their corresponding biochemical osteogenic assay results, to develop prediction model of osteogenic differentiation. Using a dataset of 9,990 images automatically acquired by BioStation CT during osteogenic differentiation culture of hBMSCs, 666 morphometric features were extracted as parameters. Two commonly used osteogenic markers, alkaline phosphatase (ALP) activity and calcium deposition were measured experimentally, and used as the true biological differentiation status to validate the prediction accuracy. Using time-course morphological features throughout differentiation culture, the prediction results highly correlated with the experimentally defined differentiation marker values (R>0.89 for both marker predictions). The clinical applicability of our morphology-based prediction was further examined with two scenarios: one using only historical cell images and the other using both historical images together with the patient's own cell images to predict a new patient's cellular potential. The prediction accuracy was found to be greatly enhanced by incorporation of patients' own cell features in the modeling, indicating the practical strategy for clinical usage. Consequently, our results provide strong evidence for the feasibility of using a quantitative time series of phase-contrast cellular morphology for non-invasive cell quality prediction in regenerative medicine.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0055082
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000315186000006&DestApp=WOS_CPL
URL
https://nitech.repo.nii.ac.jp/?action=repository_uri&item_id=5805
ID情報
  • DOI : 10.1371/journal.pone.0055082
  • ISSN : 1932-6203
  • Web of Science ID : WOS:000315186000006

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