2005年4月
Lysophosphatidylcholine induces tPA gene expression through CRE-dependent mechanism
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- ,
- ,
- 巻
- 329
- 号
- 1
- 開始ページ
- 71
- 終了ページ
- 77
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.bbrc.2005.01.094
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Lysophosphatidylcholine (lysoPC is implicated in the development of atherosclerosis and certain autoimmune diseases, and is reported to induce tissue-type plasminogen activator (tPA) at the protein level in endothelial cells. This study was designed to investigate the effect of lysoPC on tPA gene expression and the underlying molecular mechanisms in cultured endothelial cells. LysoPC transiently induced the mRNA expression of tPA in endothelial cells. LysoPC also induced the mRNA expression of urokinase-type plasminogen activator (uPA), uPA receptor, and plasminogen activator inhibitor-1, but the kinetics were different from that of tPA. Promoter analysis revealed that the cyclic AMP-responsive element of the tPA gene (tPACRE) is required for lysoPC-induced tPA expression. Furthermore, an electrophoresis mobility shift assay showed that lysoPC increased the binding activity of CRE binding protein to tPACRE. These results indicated that lysoPC transcriptionally upregulated the gene expression of tPA in endothelial cells, at least in part, via tPACRE activation. (C) 2005 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bbrc.2005.01.094
- ISSN : 0006-291X
- Web of Science ID : WOS:000227415700012