2006年7月
Microtubule-bundling activity of APC is stimulated by interaction with PSD-95
NEUROSCIENCE LETTERS
- ,
- ,
- 巻
- 403
- 号
- 1-2
- 開始ページ
- 68
- 終了ページ
- 72
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.neulet.2006.04.045
- 出版者・発行元
- ELSEVIER IRELAND LTD
Adenomatous polyposis coli (APC) tumor suppressor protein binds to microtubules, leading to microtubule bundling and stabilization. The protein also interacts with postsynaptic density (PSD)-95, a major scaffolding protein in neurons. Here, we analyzed the effects of PSD-95 on the microtubule-bundling activity of APC. The coexpression of APC and PSD-95 in COS-7 cells enhanced microtubule-bundle formation compared with the expression of APC alone. A mutant APC variant that does not associate with PSD-95 did not enhance microtubule bundling, despite coexpression with PSD-95. Immunoelectron microscopy showed that the APC-PSD-95 complex sometimes colocalized on microtubules in processes of cultured neurons. These results suggest that the microtubule-bundling activity of APC is regulated by its interaction with PSD-95, which might modulate microtubule architecture and dynamics in neurons. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.neulet.2006.04.045
- ISSN : 0304-3940
- Web of Science ID : WOS:000239611500014