論文

査読有り 筆頭著者
2003年1月

Purification and characterization of a novel extracellular β-1,3-glucanase produced byBacillus clausii NM-1 isolated from ezo abaloneHaliotis discus hannai

Journal of Bioscience and Bioengineering
  • Nobumitsu Miyanishi
  • ,
  • Naoko Hamada
  • ,
  • Takeshi Kobayashi
  • ,
  • Chiaki Imada
  • ,
  • Etsuo Watanabe

95
1
開始ページ
45
終了ページ
51
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/s1389-1723(03)80147-0
出版者・発行元
Elsevier BV

A novel extracellular alkaline stable β-1,3-glucanase produced by Bacillus clausii NM-1 isolated from the ezo abalone Haliotis discus hannai was purified by ammonium sulfate precipitation, DEAE-Sepharose FF ion exchange chromatography and Sephacryl S-200HR gel filtration. The molecular weight of the purified enzyme was estimated to be 71 kDa from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was very stable at pH 5.3 to 11.5 but unstable at pH 4.0 to 4.5. The optimum temperature and thermostability of the enzyme increased in the presence of CaCl2. The enzyme hydrolyzed β-1,3-glucan from marine organisms, but did not show activity against any other β-1,3-glucans. The major hydrolysis products of β-1,3-glucan from Laminaria digitata and Eisenia bicyclis were laminaritriose and laminaritetraose, respectively. The N-terminal amino acid sequence of the purified enzyme was similar to that of several β-1,3-glucanases in the glycoside hydrolase family 16.

リンク情報
DOI
https://doi.org/10.1016/s1389-1723(03)80147-0
ID情報
  • DOI : 10.1016/s1389-1723(03)80147-0
  • ISSN : 1389-1723
  • SCOPUS ID : 0037275925

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