2003年1月
Induction of TNF-.ALPHA. Production from Human Peripheral Blood Monocytes with .BETA.-1,3-Glucan Oligomer Prepared from Laminarin with .BETA.-1,3-Glucanase from Bacillus clausii NM-1.
Journal of Bioscience and Bioengineering
- ,
- ,
- ,
- 巻
- 95
- 号
- 2
- 開始ページ
- 192
- 終了ページ
- 195
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/s1389-1723(03)80128-7
- 出版者・発行元
- The Society for Biotechnology, Japan
We prepared a β-1,3-glucan oligomer (DP≥4) from laminarin (DP: 25–30) derived from Laminaria digitata with β-1,3-glucanase, and examined its effect on human peripheral blood monocytes. Conditioned medium prepared by incubating monocytes (MC-CM) with the β-1,3-glucan oligomer showed strong inhibitory activity against the proliferation of human leukemic U937 cells. Since the β-1,3-glucan oligomer had no direct cytotoxic effect on U937 cells up to 1000 μg/ml, the cytotoxicity of the MC-CM may be due to cytotoxic cytokines produced from monocytes stimulated by the β-1,3-glucan oligomer. On the other hand, the MC-CM prepared with original laminarin had little effect on the growth of U937 cells. The cytotoxicity of the MC-CM prepared with the β-1,3-glucan oligomer was significantly reduced by an anti-TNF-α antibody, but the anti-TNF-β antibody had no effect. Our results suggest that the enzymatically depolymerized β-1,3-glucan oligomer induces TNF-α production from human monocytes.<br>
- リンク情報
- ID情報
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- DOI : 10.1016/s1389-1723(03)80128-7
- ISSN : 1389-1723
- CiNii Articles ID : 130004145495