論文

査読有り
2003年1月

Induction of TNF-.ALPHA. Production from Human Peripheral Blood Monocytes with .BETA.-1,3-Glucan Oligomer Prepared from Laminarin with .BETA.-1,3-Glucanase from Bacillus clausii NM-1.

Journal of Bioscience and Bioengineering
  • MIYANISHI NOBUMITSU
  • ,
  • IWAMOTO YOSHIKO
  • ,
  • WATANABE ETSUO
  • ,
  • ODA TATSUYA

95
2
開始ページ
192
終了ページ
195
記述言語
英語
掲載種別
DOI
10.1016/s1389-1723(03)80128-7
出版者・発行元
The Society for Biotechnology, Japan

We prepared a β-1,3-glucan oligomer (DP≥4) from laminarin (DP: 25–30) derived from Laminaria digitata with β-1,3-glucanase, and examined its effect on human peripheral blood monocytes. Conditioned medium prepared by incubating monocytes (MC-CM) with the β-1,3-glucan oligomer showed strong inhibitory activity against the proliferation of human leukemic U937 cells. Since the β-1,3-glucan oligomer had no direct cytotoxic effect on U937 cells up to 1000 μg/ml, the cytotoxicity of the MC-CM may be due to cytotoxic cytokines produced from monocytes stimulated by the β-1,3-glucan oligomer. On the other hand, the MC-CM prepared with original laminarin had little effect on the growth of U937 cells. The cytotoxicity of the MC-CM prepared with the β-1,3-glucan oligomer was significantly reduced by an anti-TNF-α antibody, but the anti-TNF-β antibody had no effect. Our results suggest that the enzymatically depolymerized β-1,3-glucan oligomer induces TNF-α production from human monocytes.<br>

リンク情報
DOI
https://doi.org/10.1016/s1389-1723(03)80128-7
CiNii Articles
http://ci.nii.ac.jp/naid/130004145495
URL
https://jlc.jst.go.jp/DN/JALC/00162154832?from=CiNii
ID情報
  • DOI : 10.1016/s1389-1723(03)80128-7
  • ISSN : 1389-1723
  • CiNii Articles ID : 130004145495

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