MISC

2006年9月

Cyclic phosphatidic acid is produced by autotaxin in blood

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Satomi Tsuda
  • ,
  • Shinichi Okudaira
  • ,
  • Keiko Moriya-Ito
  • ,
  • Chie Shimamoto
  • ,
  • Masayuki Tanaka
  • ,
  • Junken Aoki
  • ,
  • Hiroyuki Arai
  • ,
  • Kimiko Murakami-Murofushi
  • ,
  • Tetsuyuki Kobayashi

281
36
開始ページ
26081
終了ページ
26088
記述言語
英語
掲載種別
DOI
10.1074/jbc.M602925200
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Cyclic phosphatidic acid (cPA), an analog of lysophosphatidic acid (LPA), was previously identified in human serum. Although cPA possesses distinct physiological activities not elicited by LPA, its biochemical origins have scarcely been studied. In the present study, we assayed cPA formation from lysophosphatidylcholine in fetal bovine serum and found significant activity of transphosphatidylation that generated cPA. The cPA-producing enzyme was purified from fetal bovine serum using five chromatographic steps yielding a 100-kDa protein with cPA biosynthetic activity. Matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry of its tryptic peptides revealed that the enzyme shared identical fragments with human autotaxin, a serum lysophospholipase D that produces LPA. Western blot analysis demonstrated that the 100-kDa protein was specifically recognized by an anti-human autotaxin antibody. Moreover, recombinant rat autotaxin was found to generate cPA in addition to LPA. No significant cPA-or LPA-producing activity was detected in autotaxin-depleted serum from bovine or human prepared by immunoprecipitation with an anti-autotaxin monoclonal antibody. These results indicate that the generation of cPA and LPA in serum is mainly attributed to autotaxin.

Web of Science ® 被引用回数 : 50

リンク情報
DOI
https://doi.org/10.1074/jbc.M602925200
CiNii Articles
http://ci.nii.ac.jp/naid/80018935074
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16837466
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000240249500023&DestApp=WOS_CPL

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