論文

査読有り
2013年11月

A Strain-Specific Epitope of Enterovirus 71 Identified by Cryo-Electron Microscopy of the Complex with Fab from Neutralizing Antibody

JOURNAL OF VIROLOGY
  • Hyunwook Lee
  • ,
  • Javier O. Cifuente
  • ,
  • Robert E. Ashley
  • ,
  • James F. Conway
  • ,
  • Alexander M. Makhov
  • ,
  • Yoshio Tano
  • ,
  • Hiroyuki Shimizu
  • ,
  • Yorihiro Nishimura
  • ,
  • Susan Hafenstein

87
21
開始ページ
11363
終了ページ
11370
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/JVI.01926-13
出版者・発行元
AMER SOC MICROBIOLOGY

Enterovirus 71 (EV71) is a picornavirus that causes outbreaks of hand, foot, and mouth disease (HFMD), primarily in the Asia-Pacific area. Unlike coxsackievirus A16, which also causes HFMD, EV71 induces severe neuropathology leading to high fatalities, especially among children under the age of 6 years. Currently, no established vaccines or treatments are available against EV71 infection. The monoclonal antibody MA28-7 neutralizes only specific strains of EV71 that have a conserved glycine at amino acid VP1-145, a surface-exposed residue that maps to the 5-fold vertex and that has been implicated in receptor binding. The cryo-electron microscopy structure of a complex between EV71 and the Fab fragment of MA28-7 shows that only one Fab fragment occupies each 5-fold vertex. A positively charged patch, which has also been implicated in receptor binding, lies within the Fab footprint. We identify the strain-specific epitope of EV71 and discuss the possible neutralization mechanisms of the antibody.

リンク情報
DOI
https://doi.org/10.1128/JVI.01926-13
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23946455
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000325863400005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1128/JVI.01926-13
  • ISSN : 0022-538X
  • eISSN : 1098-5514
  • PubMed ID : 23946455
  • Web of Science ID : WOS:000325863400005

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