2016年3月
MALDI In-Source Decay of Protein: The Mechanism of c-Ion Formation
Mass Spectrometry (Tokyo)
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プレプリント・著者最終稿
回数 : 34
- 巻
- 5
- 号
- 1
- 開始ページ
- 1
- 終了ページ
- 12
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.5702/massspectrometry.A0044
- 出版者・発行元
- 一般社団法人 日本質量分析学会
The in-source decay (ISD) phenomenon, the fragmentation at an N–Cα bond of a peptide backbone that occurs within several tens of nanoseconds in the ion-source in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS), is discussed from the standpoints of the discovery and early publications dealing with MALDI-ISD, the formation of c-ions in energy-sudden desorption/ionization methods, the formation of radical species in a MALDI, model construction for ISD, and matrix materials that are suitable for use in MALDI-ISD. The formation of c-ions derived from peptides and proteins in MALDI-ISD can be rationalized by a mechanism involving intermolecular hydrogen transfer, denoted as the "Takayama's model" by De Pauw's group (Anal. Chem. 79: 8678–8685, 2007). It should be emphasized that the model for MALDI-ISD was constructed on the basis of X-ray crystallography and scanning probe microscopy (SPM) analyses of matrix crystals, as well as the use of isotopically-labelled peptides.
- リンク情報
- ID情報
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- DOI : 10.5702/massspectrometry.A0044
- ISSN : 2187-137X
- CiNii Articles ID : 130005138385