2007年8月
Morphological and immunohistochemical changes in muscle tissue in association with mandibular distraction osteogenesis.
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons
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- 巻
- 65
- 号
- 8
- 開始ページ
- 1517
- 終了ページ
- 25
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.joms.2006.10.041
- 出版者・発行元
- W B SAUNDERS CO-ELSEVIER INC
Purpose: Studies of changes in the surrounding soft tissue in association with distraction osteogenesis in the maxillofacial region, where various different kinds of tissue are tightly packed, are rare. In this study, we performed morphological and immunohistochemical investigations of muscle tissue during mandibular distraction osteogenesis.
Materials and Methods: Japanese white rabbits were divided into 2 groups. In I group, the mandibular bone was distracted at a rate of 1 mm/day (DO group), whereas in the other group it was advanced by 10 mm all at once (0 group). The cross-section of the anterior belly of the digastric Muscle was examined using hematoxylin and eosin, periodic acid-Schiff (PAS), and proliferating cell nuclear antigen (PCNA) staining methods, as well as scanning electronmicroscopy (SEM).
Results: In the DO group, the cross-section of muscle bundle revealed no remarkable changes. in PAS staining, type 11 fibers gradually disappeared with distraction, but eventually reappeared after a period of consolidation. In the SEM photographs, striation-like laminated structures were evident in the control and the 3-mm distraction, ambiguous in the 6-mm distraction, and not evident in the 10-mm distraction. In contrast, the structure appeared to be restored in the consolidation for I week thereafter. In the PCNA immunostaining, the numbers of PCNA-positive nuclei increased during periods of distraction, but subsequently tended to decrease gradually.
Conclusions: Although minute injuries were induced in muscle fibers in association with distraction osteogenesis, it is suggested that muscle fibers regenerate starting in the distraction period and thus can adapt to the environment. (c) 2007 American Association of Oral and Maxillofacial Surgeons.
Materials and Methods: Japanese white rabbits were divided into 2 groups. In I group, the mandibular bone was distracted at a rate of 1 mm/day (DO group), whereas in the other group it was advanced by 10 mm all at once (0 group). The cross-section of the anterior belly of the digastric Muscle was examined using hematoxylin and eosin, periodic acid-Schiff (PAS), and proliferating cell nuclear antigen (PCNA) staining methods, as well as scanning electronmicroscopy (SEM).
Results: In the DO group, the cross-section of muscle bundle revealed no remarkable changes. in PAS staining, type 11 fibers gradually disappeared with distraction, but eventually reappeared after a period of consolidation. In the SEM photographs, striation-like laminated structures were evident in the control and the 3-mm distraction, ambiguous in the 6-mm distraction, and not evident in the 10-mm distraction. In contrast, the structure appeared to be restored in the consolidation for I week thereafter. In the PCNA immunostaining, the numbers of PCNA-positive nuclei increased during periods of distraction, but subsequently tended to decrease gradually.
Conclusions: Although minute injuries were induced in muscle fibers in association with distraction osteogenesis, it is suggested that muscle fibers regenerate starting in the distraction period and thus can adapt to the environment. (c) 2007 American Association of Oral and Maxillofacial Surgeons.
- リンク情報
- ID情報
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- DOI : 10.1016/j.joms.2006.10.041
- ISSN : 0278-2391
- eISSN : 1531-5053
- PubMed ID : 17656277
- Web of Science ID : WOS:000248706000013