MISC

2008年9月

Activation of peroxisome proliferator-activated receptor gamma suppresses mast cell maturation involved in allergic diseases

ALLERGY
  • M. Tachibana
  • ,
  • K. Wada
  • ,
  • K. Katayama
  • ,
  • Y. Kamisaki
  • ,
  • K. Maeyama
  • ,
  • T. Kadowaki
  • ,
  • R. S. Blumberg
  • ,
  • A. Nakajima

63
9
開始ページ
1136
終了ページ
1147
記述言語
英語
掲載種別
DOI
10.1111/j.1398-9995.2008.01677.x
出版者・発行元
WILEY-BLACKWELL

Background: Mast cells play a central role in allergic and inflammatory diseases. Several reports indicated role of peroxisome proliferator-activated receptor gamma (PPAR gamma) on mast cell function. However, there is no report about the role of PPAR gamma on differentiation of mast cells from the progenitors. In this study, we investigated the role of PPAR gamma in regulating bone marrow-derived mast cell maturation and the therapeutic implications for mast cell-related diseases such as atopic or contact dermatitis.
Methods: We used in vitro cell culture system for mast cell differentiation from bone marrow-progenitors using specific ligands and lentiviral-mediated short hairpin RNA of PPAR gamma, and in vivo murine dermatitis models.
Results: Activation of PPAR gamma inhibited the maturation of bone marrow progenitors into connective tissue-type mast cells (CTMCs) through up-regulation of GATA-4 and GATA-6 resulting in a decrease in expression of histidine decarboxylase and mast cell histamine content. In comparison, the differentiation of bone marrow progenitors into CTMCs was significantly accelerated by the knockdown of PPAR gamma expression by lentiviral-mediated short hairpin RNA. Peroxisome proliferator-activated receptor gamma ligand administration to mice inhibited the maturation of mast cells resulting in attenuation of atopic and contact dermatitis via diminishment of the number of mature mast cells.
Conclusion: Our results indicate that PPAR gamma is one of master regulators on mast cell maturation and potentially useful for the therapy in various disorders involving mast cell activation.

Web of Science ® 被引用回数 : 24

リンク情報
DOI
https://doi.org/10.1111/j.1398-9995.2008.01677.x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000258255100004&DestApp=WOS_CPL

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