MISC

2000年6月

Lack of androgen receptor transcriptional activity in human keratinocytes

JOURNAL OF DERMATOLOGICAL SCIENCE
  • S Inui
  • ,
  • S Itami
  • ,
  • HJ Pan
  • ,
  • CS Chang

23
2
開始ページ
87
終了ページ
92
記述言語
英語
掲載種別
DOI
10.1016/S0923-1811(99)00091-2
出版者・発行元
ELSEVIER SCI IRELAND LTD

Since detection of androgen receptor (AR) expression in keratinocytes by immunostaining is controversial, we investigated whether keratinocytes can act as androgen target cells using transient transfection assays. Chloramphenicol acetyltransferase (CAT) assays for the endogenous AR transcriptional activity in HaCaT keratinocytes indicated that DHT (10(-9)-10(-8) M) can induce less than 1.5-fold of mouse mammary tumor virus CAT, which is quite low, compared with 38-fold induction by 10(-7) M 1,25-dihydroxyvitamin D-3 of P450cc24-CAT. Furthermore, this low DHT-mediated induction could not be enhanced by the AR co-activators, ARA70 or ARA55. Western blotting analysis indicated that HaCaT and normal keratinocytes do not express AR protein. Transfection of exogenous AR into HaCaT keratinocytes, however, could install AR transcriptional activity, suggesting that HaCaT keratinocytes have all the necessary accessory factors for AR transcription activity. In conclusion, keratinocytes are unlikely to be target cells for androgen. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

Web of Science ® 被引用回数 : 20

リンク情報
DOI
https://doi.org/10.1016/S0923-1811(99)00091-2
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000087425100002&DestApp=WOS_CPL

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