MISC

1999年12月

Roles of testosterone in the growth of keratinocytes through bald frontal dermal papilla cells

ENDOCRINE
  • HJ Pan
  • ,
  • H Uno
  • ,
  • S Inui
  • ,
  • NO Fulmer
  • ,
  • CS Chang

11
3
開始ページ
321
終了ページ
327
記述言語
英語
掲載種別
出版者・発行元
HUMANA PRESS INC

A coculture of dermal papilla cells (DPCs) from the bald frontal scalp of stumptailed macaques with keratinocytes derived from human neonatal foreskin revealed that testosterone inhibited keratinocyte proliferation, and that the antiandrogen RU58841 abolished this response. This testosterone-induced keratinocyte growth inhibition was not observed when either type of cells was cultured alone. We also examined conditioned media from the coculture system and demonstrated the identical testosterone-induced growth inhibition on keratinocytes, and this inhibitory effect was conditioned media concentration-dependent. These results suggested that the testosterone-mediated suppression on keratinocyte proliferation might proceed through some diffusible growth mediators in conditioned media. Differential display reverse transcriptase polymerase chain reaction allowed us to isolate several genes from frontal DPCs that can be either suppressed or induced by testosterone. Supervillin, a membrane-associated, F-actin-binding protein, was identified as one of the testosterone downregulated genes in frontal DPCs, Further characterization of these testosterone-target genes may reveal the mechanism by which testosterone inhibits the growth of follicular cells in androgenetic alopecia.

Web of Science ® 被引用回数 : 14

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000085886700013&DestApp=WOS_CPL

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