論文

査読有り 責任著者
2009年8月

Functional importance of Crenarchaea-specific extra-loop revealed by an X-ray structure of a heterotetrameric crenarchaeal splicing endonuclease

NUCLEIC ACIDS RESEARCH
  • Shigeo Yoshinari
  • ,
  • Tomoo Shiba
  • ,
  • Daniel-Ken Inaoka
  • ,
  • Takashi Itoh
  • ,
  • Genji Kurisu
  • ,
  • Shigeharu Harada
  • ,
  • Kiyoshi Kita
  • ,
  • Yoh-ichi Watanabe

37
14
開始ページ
4787
終了ページ
4798
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/nar/gkp506
出版者・発行元
OXFORD UNIV PRESS

Archaeal splicing endonucleases (EndAs) are currently classified into three groups. Two groups require a single subunit protein to form a homodimer or homotetramer. The third group requires two nonidentical protein components for the activity. To elucidate the molecular architecture of the two-subunit EndA system, we studied a crenarchaeal splicing endonuclease from Pyrobaculum aerophilum. In the present study, we solved a crystal structure of the enzyme at 1.7-A resolution. The enzyme adopts a heterotetrameric form composed of two catalytic and two structural subunits. By connecting the structural and the catalytic subunits of the heterotetrameric EndA, we could convert the enzyme to a homodimer that maintains the broad substrate specificity that is one of the characteristics of heterotetrameric EndA. Meanwhile, a deletion of six amino acids in a Crenarchaea-specific loop abolished the endonuclease activity even on a substrate with canonical BHB motif. These results indicate that the subunit architecture is not a major factor responsible for the difference of substrate specificity between single- and two-subunit EndA systems. Rather, the structural basis for the broad substrate specificity is built into the crenarchaeal splicing endonuclease itself.

リンク情報
DOI
https://doi.org/10.1093/nar/gkp506
CiNii Articles
http://ci.nii.ac.jp/naid/80020499539
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19515941
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000269015400023&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/nar/gkp506
  • ISSN : 0305-1048
  • eISSN : 1362-4962
  • CiNii Articles ID : 80020499539
  • PubMed ID : 19515941
  • Web of Science ID : WOS:000269015400023

エクスポート
BibTeX RIS