2002年5月
Identification of the metal ion binding site on an RNA motif from hammerhead ribozymes using N-15 NMR spectroscopy
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
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- 巻
- 124
- 号
- 17
- 開始ページ
- 4595
- 終了ページ
- 4601
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1021/ja011520c
- 出版者・発行元
- AMER CHEMICAL SOC
An RNA oligomer, r(GGACGAGUCC), which mimics the metal ion-binding motif of hammerhead ribozymes, was shown to fold by itself into a conformation possessing a metal ion binding property which is similar to that of the intact ribozyme (Tanaka, et al. J. Am. Chem. Soc. 2000, 122, 11303-11310). To determine the metal ion-binding site of this motif at an atomic level, we synthesized a series of RNA oligomers which were selectively labeled with a N-15-labeled guanosine at each of the four guanosine residues. The N-15-chemical shift perturbation with Cd(II) ions by one-dimensional (1D) N-15 NMR spectra showed that the chemical shift of the N7 of the G7 residue, N7/G7, in the metal ion-binding motif was specifically perturbed. This is the first experimental evidence to prove that the N7/G7 binds with a Cd(II) ion.
- リンク情報
- ID情報
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- DOI : 10.1021/ja011520c
- ISSN : 0002-7863
- Web of Science ID : WOS:000175227600026