2019年10月
A novel approach for preparing disulfide-rich peptide-KLH conjugate applicable to the antibody production
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- ,
- ,
- 巻
- 83
- 号
- 10
- 開始ページ
- 1791
- 終了ページ
- 1799
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/09168451.2019.1618696
- 出版者・発行元
- TAYLOR & FRANCIS LTD
© 2019 Japan Society for Bioscience, Biotechnology, and Agrochemistry. To produce the antiserum against a small peptide, the target peptide-keyhole limpet hemocyanine (KLH) conjugate is generally used as an antigen, although the disulfide-rich peptide-KLH conjugate is still difficult to prepare. In our previous study, we have developed a preparation method of the disulfide-rich peptide-KLH conjugate, and this method was applied to produce the antiserum against a relaxin-like peptide. However, this method is limited to the synthetic peptide antigen, and is not applicable to a native or a recombinant peptide. In this study, to expand the applicability of this method to wide variety of peptides, we newly designed a novel thiol probe enabling the conjugation between various peptides and KLH, and applied it to produce the antiserum against relaxin-like peptide of a starfish Asterias amurensis. The antiserum obtained here showed high antibody-titer and good specificity, strongly suggesting that the method developed in this study is applicable to various peptides.
- リンク情報
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- DOI
- https://doi.org/10.1080/09168451.2019.1618696
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/31112075
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000469083300001&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85071715706&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85071715706&origin=inward
- ID情報
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- DOI : 10.1080/09168451.2019.1618696
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 31112075
- SCOPUS ID : 85071715706
- Web of Science ID : WOS:000469083300001