論文

査読有り
2017年4月

Polar Localization of the NIP5;1 Boric Acid Channel Is Maintained by Endocytosis and Facilitates Boron Transport in Arabidopsis Roots

PLANT CELL
  • Sheliang Wang
  • ,
  • Akira Yoshinari
  • ,
  • Tomoo Shimada
  • ,
  • Ikuko Hara-Nishimura
  • ,
  • Namiki Mitani-Ueno
  • ,
  • Jian Feng Ma
  • ,
  • Satoshi Naito
  • ,
  • Junpei Takano

29
4
開始ページ
824
終了ページ
842
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1105/tpc.16.00825
出版者・発行元
AMER SOC PLANT BIOLOGISTS

Boron uptake in Arabidopsis thaliana is mediated by nodulin 26-like intrinsic protein 5;1 (NIP5;1), a boric acid channel that is located preferentially on the soil side of the plasma membrane in root cells. However, the mechanism underlying this polar localization is poorly understood. Here, we show that the polar localization of NIP5;1 in epidermal and endodermal root cells is mediated by the phosphorylation of Thr residues in the conserved TPG (ThrProGly) repeat in the N-terminal region of NIP5;1. Although substitutions of Ala for three Thr residues in the TPG repeat did not affect lateral diffusion in the plasma membrane, these substitutions inhibited endocytosis and strongly compromised the polar localization of GFP-NIP5;1. Consistent with this, the polar localization was compromised in m subunit mutants of the clathrin adaptor AP2. The Thr-to-Ala substitutions did not affect the boron transport activity of GFP-NIP5;1 in Xenopus laevis oocytes but did inhibit the ability to complement boron translocation to shoots and rescue growth defects in nip5;1-1 mutant plants under boron-limited conditions. These results demonstrate that the polar localization of NIP5;1 is maintained by clathrin-mediated endocytosis, is dependent on phosphorylation in the TPG repeat, and is necessary for the efficient transport of boron in roots.

リンク情報
DOI
https://doi.org/10.1105/tpc.16.00825
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28341806
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000400746600021&DestApp=WOS_CPL
ID情報
  • DOI : 10.1105/tpc.16.00825
  • ISSN : 1040-4651
  • eISSN : 1532-298X
  • PubMed ID : 28341806
  • Web of Science ID : WOS:000400746600021

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