論文

査読有り
2014年3月1日

Scanning electron microscope imaging of amyloid fibrils

American Journal of Biochemistry and Biotechnology
  • Eisuke Takai
  • ,
  • Gai Ohashi
  • ,
  • Ryuichi Ueki
  • ,
  • Yoichi Yamada
  • ,
  • Jun-Ichi Fujita
  • ,
  • Kentaro Shiraki

10
1
開始ページ
31
終了ページ
39
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3844/ajbbsp.2014.31.39
出版者・発行元
Science Publications

This study demonstrated the applicability of Scanning Electron Microscopy (SEM) for the observation of amyloid fibrils without staining. As model specimens, two types of amyloid fibrils with different shapes and chemical compositions were controllably synthesized from hen lysozyme. The apparent fibril widths in the SEM images were considerably larger than the original diameters analyzed by the conventional techniques of Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM). Although this broadening, which depends on the chemical nature of the fibril, is not desirable for detailed imaging, it makes SEM sensitive to fibrils several micrometers in length and as thin as 3.5 nm. Note that the sensitivity also contributed to clearly distinguishing amyloid fibrils from salt microcrystals in SEM images. These results suggest the considerable applicability of SEM for the imaging of amyloid fibrils, even in contaminated samples. © 2014 Science Publication.

リンク情報
DOI
https://doi.org/10.3844/ajbbsp.2014.31.39

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