2017年2月
Utilizing the Carba NP test as an indicator of expression level of carbapenemase genes in Enterobacteriaceae
JOURNAL OF MICROBIOLOGICAL METHODS
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- 巻
- 133
- 号
- 開始ページ
- 35
- 終了ページ
- 39
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.mimet.2016.12.015
- 出版者・発行元
- ELSEVIER SCIENCE BV
The Carba NP test was developed to detect carbapenemase-producing Enterobacteriaceae, and uses imipenem as the reaction substrate. In Japan, IMP-6 metallo-beta-lactamase (MBL) producers, which are usually resistant to meropenem but susceptible to imipenem, and IMP-1 MBL producers, which are usually resistant to both carbapenems are prevalent. We performed the Carba NP test with IMP-6 and IMP-1 MBL producers, and both types were detected by the Carba NP test with high sensitivity. All IMP-1 MBL producers were detected by the Carba NP test, but the minimum inhibitory concentrations (MICs) of imipenem varied from 0.25 to >32 mu g/mL, and the time to positivity varied from 0 to 30 min. Time to positivity was significantly correlated with expression levels of bla(IMP-1), but not with MICs of imipenem. These results suggested that the Carba NP test can be used as a screening assay for carbapenemase gene expression levels among producers of the same type of carbapenemase. Using this approach, it is possible to determine whether the carbapenem resistance of each carbapenemase-producing Enterobacteriaceae isolate is primarily due to carbapenemase production, or to another mechanism such as outer membrane impermeability. (C) 2016 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.mimet.2016.12.015
- ISSN : 0167-7012
- eISSN : 1872-8359
- PubMed ID : 28007530
- Web of Science ID : WOS:000394197200007