Papers

Peer-reviewed
Jul, 2012

Simple and specific detection of Bordetella holmesii by using a loop-mediated isothermal amplification assay

MICROBIOLOGY AND IMMUNOLOGY
  • Nao Otsuka
  • ,
  • Shuji Yoshino
  • ,
  • Kimiko Kawano
  • ,
  • Hiromi Toyoizumi-Ajisaka
  • ,
  • Keigo Shibayama
  • ,
  • Kazunari Kamachi

Volume
56
Number
7
First page
486
Last page
489
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1111/j.1348-0421.2012.00465.x
Publisher
WILEY

A loop-mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay results were in complete agreement with the results of previously published real-time PCR assay, indicating that the former is a powerful tool for the accurate diagnosis and surveillance of B. holmesii.

Link information
DOI
https://doi.org/10.1111/j.1348-0421.2012.00465.x
J-GLOBAL
https://jglobal.jst.go.jp/en/detail?JGLOBAL_ID=201202211201524960
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22512310
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000305689200009&DestApp=WOS_CPL
ID information
  • DOI : 10.1111/j.1348-0421.2012.00465.x
  • ISSN : 0385-5600
  • eISSN : 1348-0421
  • J-Global ID : 201202211201524960
  • Pubmed ID : 22512310
  • Web of Science ID : WOS:000305689200009

Export
BibTeX RIS