2012年
Cell migration ability test for bioactive ceramics toward international standardization of ceramics for regenerative medicine
Key Engineering Materials
- 巻
- 493-494
- 号
- 開始ページ
- 836
- 終了ページ
- 839
- 記述言語
- 英語
- 掲載種別
- 研究論文(国際会議プロシーディングス)
- DOI
- 10.4028/www.scientific.net/KEM.493-494.836
International standard for test method on cell migration into a scaffold is one of the important things to evaluate the scaffold. The "cell migration" ability can divide into two parts. One is infiltration of cell suspension before in vitro cell culture on the scaffold. Another is migration of adherent cells from the edge of scaffold. The latter one could be closely related to cell/tissue migration into the scaffold when it is implanted into bone. Thus, in the present study, the cell migration ability was evaluated toward standardization of in vitro evaluation method for in vivo cell/tissue migration ability using several bioactive ceramics and composites including commercially available materials. The specimen 5 mm in diameter was placed on confluent MG63 cell layer. After 3 days incubation, the specimen was harvested, fixed and divided into two parts. Inside and outside of the scaffold were stained by Giemsa and observed by optical microscopy. In addition, the same specimen was critical point dried and observed with scanning electron microscope (SEM). From microscopic observation, MG63 cells migrated to pore walls of the specimen as well as a sidewall. Maximum migration distances were different among specimens and seemed to depend on pore structure and size as well as porosity. Similar behaviors were observed with SEM. Even relations between this test method and in vivo cell/tissue migration have not been evaluated, this test method is potentially a good method for testing cell migration ability of porous bioactive ceramics as well as other porous scaffold materials. © (2012) Trans Tech Publications.
- リンク情報
- ID情報
-
- DOI : 10.4028/www.scientific.net/KEM.493-494.836
- ISSN : 1013-9826
- ORCIDのPut Code : 35858736
- SCOPUS ID : 81555200440
- ORCIDで取得されたその他外部ID : a:1:{i:0;a:1:{s:14:"source-work-id";s:16:"0808172337758-61";}}