Yousuke Takahama - My portal

Profile Information

Affiliation: Senior Investigator, Experimental Immunology Branch, National Cancer Institute, NIH

Degree: Doctor of Philosophy in Medical Science(1988, Osaka University)

Contact information: yousuke.takahamanih.gov
J-GLOBAL ID: 200901099805236005
Researcher ID: A-5863-2010
researchmap Member ID: 5000001881

External link: https://ccr.cancer.gov/staff-directory/yousuke-takahama

Research Interests

Research Areas

Life sciences / Immunology / Thymus Biology

Research History

Education

Committee Memberships

2004 - Present

Founding Organizer, Global Thymus Network
2016 - 2019

Council, International Union of Immunological Societies
2016 - 2018

Science Adviser, MEXT
2015 - 2018

Treasurer, Federation of Immunological Societies of Asia-Oceania
2008 - 2018

Board, Japanese Society for Immunology
2013 - 2016

Program Officer, JSPS Research Center for Science Systems

Awards

2000

Japanese Society for Immunology Award

Papers

Functionally diverse thymic medullary epithelial cells interplay to direct central tolerance.

Aya Ushio, Mami Matsuda-Lennikov, Felix Kalle-Youngoue, Akihide Shimizu, Abdalla Abdelmaksoud, Michael C Kelly, Naozumi Ishimaru, Yousuke Takahama

Cell Reports, 43(4) 114072-114072, Apr 5, 2024
Developmental conversion of thymocyte-attracting cells into self-antigen-displaying cells in embryonic thymus medulla epithelium.

Izumi Ohigashi, Andrea J White, Mei-Ting Yang, Sayumi Fujimori, Yu Tanaka, Alison Jacques, Hiroshi Kiyonari, Yosuke Matsushita, Sevilay Turan, Michael C Kelly …

eLife, 12, Mar 11, 2024
Recycling of memory B cells between germinal center and lymph node subcapsular sinus supports affinity maturation to antigenic drift

Yang Zhang, Laura Garcia-Ibanez, Carolin Ulbricht, Laurence S. C. Lok, Jeremy A. Pike, Jennifer Mueller-Winkler, Thomas W. Dennison, John R. Ferdinand, Cameron J. M. Burnett, Juan C. Yam-Puc …

Nature Communications, 13(1), Dec, 2022 Peer-reviewed
An endogenous CCL21-Ser deficiency reduces melanoma growth by an enhanced antitumor immunity

Fujie R, Kurowarabe K, Yamada Y, Fujiwara K, Hayashi R, Kawahata H, Miyamoto M, Takahama Y, Ohigashi I, HAYASAKA H

Heliyon, 9 e19215, Apr, 2022
Fine-tuning of β-catenin in mouse thymic epithelial cells is required for postnatal T-cell development

Sayumi Fujimori, Izumi Ohigashi, Hayato Abe, Yosuke Matsushita, Toyomasa Katagiri, Makoto M Taketo, Yousuke Takahama, Shinji Takada

eLife, 11 e69088, Jan 19, 2022 Peer-reviewed
Radiation inducible MafB gene is required for thymic regeneration

Daiki Hashimoto, Jose Gabriel R. Colet, Aki Murashima, Kota Fujimoto, Yuko Ueda, Kentaro Suzuki, Taiju Hyuga, Hiroaki Hemmi, Tsuneyasu Kaisho, Satoru Takahashi …

Scientific Reports, 11(1) 10439-10439, Dec, 2021 Peer-reviewed
The thymoproteasome hardwires the TCR repertoire of CD8+ T cells in the cortex independent of negative selection

Izumi Ohigashi, Melina Frantzeskakis, Alison Jacques, Sayumi Fujimori, Aya Ushio, Fusano Yamashita, Naozumi Ishimaru, Da Yin, Margaret Cam, Michael C. Kelly …

The Journal of Experimental Medicine, 218(4), Apr 5, 2021 Peer-reviewed
Medullary stromal cells synergize their production and capture of CCL21 for T-cell emigration from neonatal mouse thymus

Kieran D. James, Daniel F. Legler, Vladimir Purvanov, Izumi Ohigashi, Yousuke Takahama, Sonia M. Parnell, Andrea J. White, William E. Jenkinson, Graham Anderson

Blood Advances, 5(1) 99-112, Jan 12, 2021 Peer-reviewed
PITHD1 is a proteasome-interacting protein essential for male fertilization

Hiroyuki Kondo, Takafumi Matsumura, Mari Kaneko, Kenichi Inoue, Hidetaka Kosako, Masahito Ikawa, Yousuke Takahama, Izumi Ohigashi

Journal of Biological Chemistry, 295(6) 1658-1672, Feb, 2020 Peer-reviewed
Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells

Izumi Ohigashi, Yu Tanaka, Kenta Kondo, Sayumi Fujimori, Hiroyuki Kondo, Amy C Palin, Victoria Hoffmann, Mina Kozai, Yosuke Matsushita, Shinsuke Uda …

Cell Reports, 29(9) 2901-2916, Nov 26, 2019 Peer-reviewed
TCR Affinity for In Vivo Peptide-Induced Thymic Positive Selection Fine-Tunes TCR Responsiveness of Peripheral CD8+ T Cells

Umme Shahina Khanom, Izumi Ohigashi, Sayumi Fujimori, Kenta Kondo, Kensuke Takada, Yousuke Takahama

The Journal of Immunology, 203(4) 881-887, Aug 15, 2019 Peer-reviewed
Formation of the Intrathymic Dendritic Cell Pool Requires CCL21-Mediated Recruitment of CCR7⁺ Progenitors to the Thymus

Emilie J. Cosway, Izumi Ohigashi, Karin Schauble, Sonia M. Parnell, William E. Jenkinson, Sanjiv Luther, Yousuke Takahama, Graham Anderson

The Journal of Immunology, 201(2) 516-523, Jul 15, 2018 Peer-reviewed
Cellularity of Thymic Epithelial Cells in the Postnatal Mouse

Mie Sakata, Izumi Ohigashi, Yousuke Takahama

The Journal of Immunology, Vol.200(No.4) 1382-1388, Feb 15, 2018 Peer-reviewed
MiCASA is a new method for quantifying cellular organization

Andrew Sornborger, Jie Li, Cullen Timmons, Floria Lupu, Jonathan Eggenschwiler, Yousuke Takahama, Nancy R. Manley

Nature Communications, 8(1), Aug, 2017 Peer-reviewed
Essential role of CCL21 in establishment of central self-tolerance in T cells

Mina Kozai, Yuki Kubo, Tomoya Katakai, Hiroyuki Kondo, Hiroshi Kiyonari, Karin Schaeuble, Sanjiv A. Luther, Naozumi Ishimaru, Izumi Ohigashi, Yousuke Takahama

The Journal of Experimental Medicine, Vol.214(No.7) 1925-1935, Jul 3, 2017 Peer-reviewed
A human PSMB11 variant affects thymoproteasome processing and CD8+ T cell production

Izumi Ohigashi, Yuki Ohte, Kazuya Setoh, Hiroshi Nakase, Akiko Maekawa, Hiroshi Kiyonari, Yoko Hamazaki, Miho Sekai, Tetsuo Sudo, Yasuharu Tabara …

JCI Insight, Vol.2(No.10), May 18, 2017 Peer-reviewed
Foxn1-beta5t transcriptional axis controls CD8+ T-cell production in the thymus

MM Uddin, Izumi Ohigashi, R Motosugi, T Nakayama, M Sakata, J Hamazaki, Y Nishito, I Rode, K Tanaka, Tatsuya Takemoto …

Nature Communications, Vol.8 14419-14419, Feb 8, 2017 Peer-reviewed
Dynamic spatio-temporal contribution of single β5t+ cortical epithelial precursors to the thymus medulla

Carlos E. Mayer, Saulius Žuklys, Saule Zhanybekova, Izumi Ohigashi, Hong-Ying Teh, Stephen N. Sansom, Noriko Shikama-Dorn, Katrin Hafen, Iain C. Macaulay, Mary E. Deadman …

European Journal of Immunology, 46(4) 846-856, Apr, 2016 Peer-reviewed
Adult thymic medullary epithelium is maintained and regenerated by lineage-restricted cells rather than bipotent progenitors

Izumi Ohigashi, Saulius Zuklys, Mie Sakata, Carlos E. Mayer, Yoko Hamazaki, Nagahiro Minato, Georg A Hollander, Yousuke Takahama

Cell Reports, Vol.13(No.7) 1432-1443, Nov 17, 2015 Peer-reviewed
Thymoproteasomes produce unique peptide motifs for positive selection of CD8+ T cells

Katsuhiro Sasaki, Kensuke Takada, Yuki Ohte, Hiroyuki Kondo, Hiroyuki Sorimachi, Keiji Tanaka, Yousuke Takahama, Shigeo Murata

Nature Communications, 6(1) 7484-7484, Nov, 2015 Peer-reviewed

Misc.

The thymoproteasome in shaping the CD8+ T-cell repertoire.

Yousuke Takahama

Current Opinion in Immunology, 83 102336-102336, Aug, 2023
Large-Scale Isolation of Mouse Thymic Epithelial Cells.

Izumi Ohigashi, Mami Matsuda-Lennikov, Yousuke Takahama

Methods in Molecular Biology (Clifton, N.J.), 2580 189-197, 2023
Tissue-specific proteasomes in generation of MHC class I peptides and CD8+ T cells.

Mami Matsuda-Lennikov, Izumi Ohigashi, Yousuke Takahama

Current Opinion in Immunology, 77 102217-102217, Aug, 2022
mTEC damage risks immune recovery

Yousuke Takahama

Journal of Experimental Medicine, 219(2), Feb 7, 2022
Peptides for T cell selection in the thymus

Izumi Ohigashi, Mami Matsuda-Lennikov, Yousuke Takahama

Peptides, 146 170671-170671, Dec, 2021
Specific impact of β5t on proteasome subunit composition in cortical thymic epithelial cells

Izumi Ohigashi, Yousuke Takahama

Cell Reports, 36(10) 109657-109657, Sep, 2021
The Role of Proteasomes in the Thymus

Melina Frantzeskakis, Yousuke Takahama, Izumi Ohigashi

Frontiers in Immunology, 12, Mar 19, 2021
Thymoproteasome optimizes positive selection of CD8+ T cells without contribution of negative selection

Izumi Ohigashi, Yousuke Takahama

Advances in Immunology, 1-23, 2021
Postnatal Involution and Counter-Involution of the Thymus

Jennifer E. Cowan, Yousuke Takahama, Avinash Bhandoola, Izumi Ohigashi

Frontiers in Immunology, 11, May 12, 2020
Thymus machinery for T-cell selection

Kenta Kondo, Izumi Ohigashi, Yousuke Takahama

International Immunology, 31(3) 119-125, Mar 5, 2019
Biology, evolution, and history of antigen processing and presentation

Masanori Kasahara, Martin F. Flajnik, Yousuke Takahama

Immunogenetics, 71(3) 137-139, Mar, 2019
Thymoproteasome and peptidic self

Yousuke Takahama, Izumi Ohigashi, Shigeo Murata, Keiji Tanaka

Immunogenetics, 71(3) 217-221, Mar, 2019
The immunoproteasome and thymoproteasome: functions, evolution and human disease

Shigeo Murata, Yousuke Takahama, Masanori Kasahara, Keiji Tanaka

Nature Immunology, 19(9) 923-931, Sep, 2018
T細胞の自己寛容性確立におけるCCL21の役割

大東いずみ, 石丸直澄, 片貝智哉, 高浜洋介

生命科学系学会合同年次大会, 2017年度 [4P2T24-06(3P, Dec, 2017
Antigen processing and presentation in the thymus: implications for T cell repertoire selection

Kenta Kondo, Kensuke Takada, Yousuke Takahama

CURRENT OPINION IN IMMUNOLOGY, 46 53-57, Jun, 2017
Generation of diversity in thymic epithelial cells

Yousuke Takahama, Izumi Ohigashi, Song Baik, Graham Anderson

NATURE REVIEWS IMMUNOLOGY, 17(5) 295-305, May, 2017
Generation of Peptides That Promote Positive Selection in the Thymus

Kensuke Takada, Kenta Kondo, Yousuke Takahama

JOURNAL OF IMMUNOLOGY, 198(6) 2215-2222, Mar, 2017
Development and developmental potential of cortical thymic epithelial cells

Izumi Ohigashi, Mina Kozai, Yousuke Takahama

IMMUNOLOGICAL REVIEWS, 271(1) 10-22, May, 2016
Flow Cytometry Analysis of Thymic Epithelial Cells and Their Subpopulations

Izumi Ohigashi, Yousuke Takahama

T-Cell Development, 65-73, 2016
Positive-Selection-Inducing Self-Peptides Displayed by Cortical Thymic Epithelial Cells

Kensuke Takada, Yousuke Takahama

Advances in Immunology, 87-110, 2015

Books and Other Publications

Synthetic Immunology

Watanabe, T, Takahama, Y

Springer, 2016 (ISBN: 4431560254)
Thymic development and selection of T lymphocytes

Boehm T, Takahama Y

Springer, 2014 (ISBN: 9783642402517)
Your amazing immune system

Takahama Y, Kawamoto H, Miyake S, Ohteki T, Sorimachi N, Taki S, Ishikawa T, Miyasaka M

Japanese Society for Immunology and European Federation of Immunological Societies, 2008

Professional Memberships

1993 - Present

American Association of Immunologists
1995 - 2019

Molecular Biology Society of Japan
1982 - 2019

Japanese Society for Immunology

Research Projects

Positive selection of T cells in the thymic cortex

JSPS, 2016 - 2019
Positive selection dictates T cell-mediated body defense

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2017

Takada Kensuke, Takahama Yousuke
Development and function of thymic microenvironments

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Jun, 2012 - Mar, 2017

TAKAHAMA Yousuke
Analysis and Synthesis of Multidimensional Immune Organ Network

MEXT, 2012 - 2017
Molecular basis for the thymic microenvironments that characterize the immune system

JSPS, 2011 - 2014
The challenge for the development of therapy for autoimmune disorder by the establishment of artificial thymic medullary organ

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, 2011 - 2012

OHIGASHI Izumi, TAKAHAMA Yousuke, NITTA Takeshi
Roles of lymph node stromal cell network in T cell homeostasis

Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, 2010 - 2011

TAKADA Kensuke, TAKAHAMA Yousuke
Immunological Self Recognition and its Disorders

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Priority Areas, Japan Society for the Promotion of Science, 2007 - 2011

MINATO Nagahiro
Thymic microenvironments for shaping T cell repertoire

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Priority Areas, MEXT, 2007 - 2011

TAKAHAMA Yousuke, MATSUMOTO Mitsuru
胸腺プロテアソームの機能

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2009 - 2010

新田剛, 高浜洋介
Tリンパ球再選択による自己免疫疾患治療法開発への挑戦

科学研究費助成事業, 挑戦的萌芽研究, 日本学術振興会, 2009 - 2010

高濱洋介, 新田剛
Molecular mechanisms that regulate the formation of the thymic microenvironments

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2008 - 2010

TAKAHAMA Yousuke, NITTA Takeshi, UENO Tomoo
Cellular dyntarnics and migration during T-Iympharyle development and selection in the thymus

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2006 - 2007

TAKAHAMA Yousuke
胸腺器官発生機構の包括的解析

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2006 - 2007

岩波礼将, 高浜洋介
胸腺の器官形成を担う分子ネットワークのマスターマップ作成

科学研究費助成事業, 萌芽研究, 日本学術振興会, 2006 - 2006

高濱洋介
生体防御と免疫病制御の統合的パラダイム

科学研究費助成事業, 基盤研究(C), 日本学術振興会, 2006 - 2006

湊長博, 清野宏, 斉藤隆, 高濱洋介, 山本一彦, 渡邊武
The roles of HIF-1α in T cell-dependent immune responses

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2005 - 2006

TOMITA Shuhei, TAKAHAMA Yousuke
Thymocyte dynamics and hymus organogenesis

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Priority Areas, Japan Society for the Promotion of Science, 2003 - 2006

TAKAHAMA Yousuke, TOMITA Shuhei
Dynamism of immune cells in immune-tissue formation

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Priority Areas, Japan Society for the Promotion of Science, 2003 - 2006

MATSUSHIMA Kouji
Microenvironments for thymocyte development

MEXT, 2003 - 2006