2003年11月
Gene cloning and function analysis of replication factor C from Thermococcus kodakaraensis KOD1
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 67
- 号
- 11
- 開始ページ
- 2373
- 終了ページ
- 2380
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1271/bbb.67.2373
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Replication factor C (RFC) catalyzes the assembly of circular proliferating cell nuclear antigen (PCNA) clamps around primed DNA, enabling processive synthesis by DNA polymerase. The RFC-like genes, arranged in tandem in the Thermococcus kodakaraensis KOD1 genome, were cloned individually and co-expressed in Escherichia coli cells. T. kodakaraensis KOD1 RFC homologue (Tk-RFC) consists of the small subunit (Tk-RFCS: MW = 37.2 kDa) and the large subunit (Tk-RFCL: MW = 57.2 kDa). The DNA elongation rate of the family B DNA polymerase from T. kodakaraensis KOD1 (KOD DNA polymerase), which has the highest elongation rate in all thermostable DNA polymerases, was increased about 1.7 times, when T. kodakaraensis KOD1 PCNA (Tk-PCNA) and the Tk-RFC at the equal molar ratio of KOD DNA polymerase were reacted with primed DNA.
- リンク情報
- ID情報
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- DOI : 10.1271/bbb.67.2373
- ISSN : 0916-8451
- eISSN : 1347-6947
- Web of Science ID : WOS:000186888100009