MISC

2009年5月

Fully phosphorylated fetuin-A forms a mineral complex in the serum of rats with adenine-induced renal failure

KIDNEY INTERNATIONAL
  • Isao Matsui
  • ,
  • Takayuki Hamano
  • ,
  • Satoshi Mikami
  • ,
  • Naohiko Fujii
  • ,
  • Yoshitsugu Takabatake
  • ,
  • Yasuyuki Nagasawa
  • ,
  • Noritaka Kawada
  • ,
  • Takahito Ito
  • ,
  • Hiromi Rakugi
  • ,
  • Enyu Imai
  • ,
  • Yoshitaka Isaka

75
9
開始ページ
915
終了ページ
928
記述言語
英語
掲載種別
DOI
10.1038/ki.2008.700
出版者・発行元
NATURE PUBLISHING GROUP

The serum glycoprotein fetuin-A is an important inhibitor of extra-osseous calcification, but correlations between serum fetuin-A levels and the extent of vascular calcification are controversial. In this study, we used a rat model of adenine-induced renal failure with secondary hyperparathyroidism that exhibits all characteristic features of patients with chronic kidney disease. These rats had medial vascular calcification along with reduced levels of both serum and hepatic fetuin-A. Treatment with an inhibitor of ectopic calcification, alendronate, decreased bone turnover and eliminated completely the vascular calcification in this rat model, but there was no change in the levels of hepatic and serum fetuin-A. Centrifugation of the serum of untreated rats with renal failure gave a small precipitate composed of fetuin-A, calcium, magnesium, and phosphate; this complex, absent from normal rat serum, was not found in the serum of alendronate-treated rats with renal failure. Rat serum contained three types of phosphorylated fetuin-A, as well as unphosphorylated forms, but only the fully phosphorylated fetuin-A was present in the mineral complex. The amount of this complex reflected the risk of mineral precipitation. Our results suggest that the measurement of serum fetuin-mineral complex rather than fetuin-A alone might provide a better indication of extra-osseous calcification propensity.

Web of Science ® 被引用回数 : 88

リンク情報
DOI
https://doi.org/10.1038/ki.2008.700
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000265220900009&DestApp=WOS_CPL

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