MISC

2009年4月

Histological Reaction of Sintered Nanohydroxyapatite-Coated Cuff and Its Fibroblast-Like Cell Hybrid for an Indwelling Catheter

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
  • Tsutomu Furuzono
  • ,
  • Mitsuki Ueki
  • ,
  • Harumi Kitamura
  • ,
  • Kazumasa Oka
  • ,
  • Enyu Imai

89B
1
開始ページ
77
終了ページ
85
記述言語
英語
掲載種別
DOI
10.1002/jbm.b.31189
出版者・発行元
WILEY-LISS

Rapid tissue-ingrowth of a sintered hydroxyapatite(HAp)-coated and cell-hybrid subcutaneous cuff equipped with an indwelling catheter was developed. The rod-like HAp nanoparticles were coated by covalent bonding on the surface of the silk fibroin (SF) fibers for about 100 mu m of the length. The fibers were transplanted three-dimensionally on a cuff substrate made of silicone elastomer with an adhesive. The fibroblast-like cells, explanted and proliferated from skin tissue containing the epidermis, dermis, and subcutaneous tissue of Japanese white rabbits, were incubated on the three-dimensional cuff for three days. Three types of cuff-polyester, HAp-coated, and cell-hybrid cuffs-were percutaneously implanted into the backs of the same animals for 3 and 7 days. The subcutaneous tissues around the cuff's were stained with hematoxylin-eosin. Immunohistochemical staining to identify macrophages and alpha-smooth muscle actin (alpha-SMA) was also done and examined by light microscopy. The alpha-SMA-positive area was very limited in the polyester cuff group even after 7 days, although many macrophages infiltrated into the fibers. In the cell-hybrid cuff group, on the other hand, an alpha-SMA-positive area was formed extensively after 3 and 7 days, causing severe inflammation. In the HAp-coated cuff group, an alpha-SMA-positive area was formed among the fibers with little inflammation. The extent order of the a-SMA-positive area was cell-hybrid cuff >> HAp-coated cuff >> polyester cuff, while the degree of inflammatory cells order was cell-hybrid cuff >> polyester cuff >> HAp-coated cuff. (C) 2008 Wiley Periodicals. Inc. J Biomed Mater Res Part B: Appl Biomater 89B: 77-85, 2009

Web of Science ® 被引用回数 : 20

リンク情報
DOI
https://doi.org/10.1002/jbm.b.31189
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000264460200009&DestApp=WOS_CPL

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