2020年6月
Generation of Footprint-Free Canine Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus Vector.
Molecular reproduction and development
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- 巻
- 87
- 号
- 6
- 開始ページ
- 663
- 終了ページ
- 665
- 記述言語
- 英語
- 掲載種別
- 研究論文（学術雑誌）
- DOI
- 10.1002/mrd.23349
- 出版者・発行元
- MARY ANN LIEBERT, INC
Using auto-erasable Sendai virus vector, we generated ciPSC line. After several passages, virus was not present in ciPSCs by RT-PCR. ciPSCs from canine PBMCs had pluripotent state, differentiated all three germ layers in vitro, and had normal 78 XX karyotype. These results proved that PBMCs were one of the good cell sources to generate ciPSC lines from companion and patient dogs.
- ID情報
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- DOI : 10.1002/mrd.23349
- ISSN : 1557-8534
- PubMed ID : 32424848