論文

査読有り 国際誌
2020年6月

Generation of Footprint-Free Canine Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus Vector.

Molecular reproduction and development
  • Masaya Tsukamoto
  • ,
  • Kazuto Kimura
  • ,
  • Miyuu Tanaka
  • ,
  • Mitsuru Kuwamura
  • ,
  • Manami Ohtaka
  • ,
  • Mahito Nakanishi
  • ,
  • Kikuya Sugiura
  • ,
  • Shingo Hatoya

87
6
開始ページ
663
終了ページ
665
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/mrd.23349
出版者・発行元
MARY ANN LIEBERT, INC

Using auto-erasable Sendai virus vector, we generated ciPSC line. After several passages, virus was not present in ciPSCs by RT-PCR. ciPSCs from canine PBMCs had pluripotent state, differentiated all three germ layers in vitro, and had normal 78 XX karyotype. These results proved that PBMCs were one of the good cell sources to generate ciPSC lines from companion and patient dogs.

リンク情報
DOI
https://doi.org/10.1002/mrd.23349
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32424848

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