論文

査読有り 国際誌
2020年3月13日

Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor.

Nature communications
  • Hirokazu Yagi
  • ,
  • Maho Yagi-Utsumi
  • ,
  • Rena Honda
  • ,
  • Yusaku Ohta
  • ,
  • Taiki Saito
  • ,
  • Miho Nishio
  • ,
  • Satoshi Ninagawa
  • ,
  • Kousuke Suzuki
  • ,
  • Takahiro Anzai
  • ,
  • Yukiko Kamiya
  • ,
  • Kazuhiro Aoki
  • ,
  • Mahito Nakanishi
  • ,
  • Tadashi Satoh
  • ,
  • Koichi Kato

11
1
開始ページ
1368
終了ページ
1368
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41467-020-15192-1

MCFD2 and ERGIC-53, which are the products of causative genes of combined factor V and factor VIII deficiency, form a cargo receptor complex responsible for intracellular transport of these coagulation factors in the early secretory pathway. In this study, using an NMR technique, we successfully identified an MCFD2-binding segment from factor VIII composed of a 10 amino acid sequence that enhances its secretion. This prompted us to examine possible effects of attaching this sequence to recombinant glycoproteins on their secretion. We found that the secretion level of recombinant erythropoietin was significantly increased simply by tagging it with the passport sequence. Our findings not only provide molecular basis for the intracellular trafficking of coagulation factors and their genetic deficiency but also offer a potentially useful tool for increasing the production yields of recombinant glycoproteins of biopharmaceutical interest.

リンク情報
DOI
https://doi.org/10.1038/s41467-020-15192-1
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32170195
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069976

エクスポート
BibTeX RIS