FUJISAWA Tetsuro

J-GLOBAL         Last updated: Jul 2, 2019 at 02:42
 
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Name
FUJISAWA Tetsuro
Affiliation
Gifu University
Section
Faculty of Engineering
Job title
Professor
Degree
Doctor of Engineering(Osaka University)
Other affiliation
Nagoya University

Profile

Solution structure of protein complex by synchrotron SAXS/Recovery from protein aggregates using hydrostatic pressure

Research Areas

 

Academic & Professional Experience

 
 
   
 
Professor, Gifu University Department of Biomolecular Science, Graduate School of Engineering
 
2007
   
 
Professor of Gifu University
 
1990
 - 
1997
Scientist of RIKEN
 
1998
 - 
2007
Senior Scientist of RIKEN
 
2007
 - 
Today
Professor of Gifu University
 

Education

 
 
 - 
1989
Biophysical Engineering, Graduate School of Engineering Science, Osaka University
 
 
 - 
1984
Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University
 

Published Papers

 
Tsuyoshi Konuma, Kazumasa Sakurai, Masanori Yagi, Yuji Goto, Tetsuro Fujisawa, Tetsuro Fujisawa, Satoshi Takahashi
Journal of Molecular Biology   427(19) 3158-3165   Apr 2015   [Refereed]
© 2015 Elsevier Ltd. All rights reserved. In the folding of β-lactoglobulin (βLG), a predominantly β-sheet protein, a transient intermediate possessing an excess amount of non-native α-helix is formed within a few milliseconds. To characterize the...
Ryo Ishiguro, Ryo Ishiguro, Hiroshi Matsuo, Keiichi Kameyama, Hideki Tachibana, Tetsuro Fujisawa, Tetsuro Fujisawa
Electrophoresis   36(6) 893-901   Mar 2015   [Refereed]
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. A disulfide-deficient variant of hen lysozyme, 0SS, is known to form an amyloid protofibril spontaneously, and to dissociate into monomers at high hydrostatic pressure. We carried out native PAGE ...
Tetsuro Fujisawa
Sub-Cellular Biochemistry   72 663-675   Jan 2015   [Refereed]
© Springer Science+Business Media Dordrecht 2015. Small-angle scattering, solution scattering from proteins in solution, reflects the shape of the scatter as a spread of electron density, which is common to protein crystallography. Although the ob...
Yuichi Shigeoka, Tetsuro Fujisawa, Satoshi Teshiba, Hisayoshi Fukumori, Kohji Yamamoto, Yutaka Banno, Yoichi Aso
Bioscience, Biotechnology and Biochemistry   77(8) 1637-1644   Sep 2013   [Refereed]
The Bacillus stearothermophilus lipoate acetyltransferase (E2), composed of sixty identical, subunits is the core component of the pyruvate dehydrogenase complex (PDC). E2 polypeptide is composed of LD, PSBD, and CD domains. Most studies had focus...
小角散乱データからのモデリングに関する出版ガイドラインを読み解く
Fujisawa Tetsuro(Gifu Univ.)
26 214-226   Jul 2013

Misc

 
Toshihisa Tanaka, Masahiro Fujita, Akihisa Takeuchi, Yoshio Suzuki, Kentaro Uesugi, Kazuki Ito, Tetsuro Fujisawa, Yoshiharu Doi, Tadahisa Iwata
Polymer Preprints, Japan   54 5290-5291   Dec 2005
Biodegradable high-strength fibers of poly[(R)-3-hydroxybutyrate] and poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate] were prepared by one-step-drawing after isothermally crystallization near the glass temperature. This drawing technique is a...
Toshihisa Tanaka, Masahiro Fujita, Akihisa Takeuchi, Yoshio Suzuki, Kentaro Uesugi, Tetsuro Fujisawa, Yoshiharu Doi, Tadahisa Iwata
Polymer Preprints, Japan   54 656   Dec 2005
Biodegradable poly(L-lactic acid) (PLLA) fibers were prepared from commercial pellet by melting spinning at 185°C. The extruder was taken up at room temperature by the roller to obtain amorphous fiber. The changes of molecular structures and highe...
Tomohiro Matsufuji, Yuji Jinbo, Yoshinobu Izumi, Hidenori Yoshino, Tetsuro Fujisawa
Polymer Preprints, Japan   54 5041   Dec 2005
Stopped-flow small-angle X-ray scattering (SF-SAXS) was used to characterize a transient intermediate in the calcium dissociation from calmodulin (CaM) complexed with a target peptide (MLCKp or RS20). Reactions were monitored by SF-SAXS instrument...
Tomoharu Sawayanagi, Masahiro Fujita, Toshihisa Tanaka, Tadahisa Iwata, Hideki Abe, Hideki Abe, Yoshiharu Doi, Yoshiharu Doi, Kazuki Ito, Tetsuro Fujisawa
Polymer Preprints, Japan   54 655   Dec 2005
Poly[(R)-3-hydroxybutyrate-co-(R)-16.0mol%3-hydroxyvalerate] single crystals were grown isothermally from an 1-octanol solution. Annealing behavior of the single crystals were followed in real time by synchrotron small- (SAXS) and wide-angle X-ray...
Masahiro Fujita, Tomoharu Sawayanagi, Toshihisa Tanaka, Tadahisa Iwata, Hideki Abe, Hideki Abe, Yoshiharu Doi, Yoshiharu Doi, Kazuki Ito, Tetsuro Fujisawa
Polymer Preprints, Japan   54 654   Dec 2005
Poly[(R)-3-hydroxybutyrate] (P(3HB)) single crystals were grown isothermally at 60, 80, and 100°C in dilute 1-octanol solution. Annealing behavior of the sedimented single crystal mats was followed in real time by synchrotron small- (SAXS) and wid...

Books etc

 
X線・放射光の分光
講談社サイエンティフィック   2009   
Trends in high pressure bioscience and biotechnology
T. Fujisawa, Y. Nishikawa, Y. Inoko (Part:Joint Work, The use of a small-angle X-ray scattering technique with a third-generation synchrotoron X-ray source in high pressure science)
2002   
Biological systems under extreme conditions
T. Fujisawa, M. Kato (Part:Joint Work, The small-angle x-ray scattering from porteins under pressure)
Elsevier Science   2001   
The use of a small-angle X-ray scattering technique with a third-generation synchrotoron X-ray source in high pressure science
Elsevier Science, Amsterdam, the Netherlands   2002   
Portable Synchrotron Light Sources and Advanced Applications, AIP conference Proceedings
American Institute of Physics   2007   

Conference Activities & Talks

 
Synchrotron small-angle x-rayscattering studies on protein complexes under high hydrostatic pressure [Invited]
Fujisawa Tetsuro (Gifu Univ)
Sep 2012   
高圧X線小角散乱法を用いた細胞骨格繊維タンパク質の安定性の研究
Popp David(Insititute of Molecular and Cell Biology, Singapore)
Sep 2011   
アクチン相同骨格タンパク質フィラメントMreB、Alfaの高圧X線小角散乱による研究
Popp David(IMCB,SIngapore)
Sep 2010   
X線小角散乱法による、gelsolin、villinのCa2+結合に伴う構造変化の解析
Chumnarnsilpa Sakesit(Institute of Molecular and Cell Biology, Singapore),Robinson Robert(Institute of Molecular and Cell Biology, Singapore)
May 2009   
Nucleotide caps and the stability of cytoskeletal fibrous proteins
Fujisawa Tetsuro(Gifu Univ.),Matsuo Hiroshi(RIKEN Harima),Iwasa Mitsusada(ERATO),Erickson Harold(Duke Univ),Kamimura Shinji(Chuo Univ),Maeda Yuichiro(ERATO),Popp David(ERATO)
Dec 2008   

Research Grants & Projects

 
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2008 - 2010    Investigator(s): Tetsuro FUJISAWA
We improved our own designed synchrotron high-pressure jump small-angle scattering system, by which stabilities on various cytoskeletal fibrous proteins were explored. Actin homologue proteins, ParM, FtsZ, Alfa, MreB were studied, and the result w...
Protein folding dynamics by use of small-angle x-ray scattering
0086 (Japanese Only)
Project Year: 1998 - 2004
Application of hydrostatic pressure on Biology