2017年3月
Isolation of equine herpesvirus 3 (EHV-3) from equine coital exanthema of two stallions and sero-epidemiology of EHV-3 infection in Japan
JOURNAL OF VETERINARY MEDICAL SCIENCE
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- 巻
- 79
- 号
- 3
- 開始ページ
- 636
- 終了ページ
- 643
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1292/jvms.16-0518
- 出版者・発行元
- JAPAN SOC VET SCI
In the spring of 2015, two stallions reared in Farms A and B in Hokkaido in Japan showed symptoms of equine coital exanthema. Equine herpesvirus 3 (EHV-3) was isolated from penis swab samples of both stallions, and the isolates from each stallion in Farms A and B were designated as SS-1 and YS-1 strains, respectively. BamHl restriction profiles of SS-1 and Japanese reference strain lwate-1 were indistinguishable, but the BamHl-A fragment of YS-1 was larger than those of SS-1 and lwate-1 by 1.9 kbp because of the lack of two BamHI sites. Nucleotide sequence analyses of glycoprotein G (gG), gB, gC and VP13/14 coding regions revealed that 55-1 and YS-1 had 99.77% to 100% identities to each other. These results suggested that the origins of SS-1 and YS-1 were different. For a sero-epidemiological survey, serum neutralizing tests using SS-1 against 319 sera of horses from eight farms in Hokkaido were conducted. Six of the eight farms were EHV-3 antibody-positive, and positive rates ranged from 2.6% to 17.6%. To determine the infection time of four EHV-3 antibody-positive horses, a retrospective study was conducted. Infection time of the four horses was in the breeding season, and re-infection or reactivation of latently infected EHV-3 might have occurred in one horse. However, these four horses had never shown any clinical symptoms. The results suggested that several EHV-3 strains are distributed in Japan and that infection is maintained widely in horses without clinical symptoms.
- リンク情報
- ID情報
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- DOI : 10.1292/jvms.16-0518
- ISSN : 0916-7250
- eISSN : 1347-7439
- PubMed ID : 28132964
- Web of Science ID : WOS:000397830400031