2015年7月
Novel pH-Responsive Polymeric Micelles Prepared through Self-assembly of Amphiphilic Block Copolymer with Poly-4-vinylpyridine Block Synthesized by Mechanochemical Solid-State Polymerization
CHEMICAL & PHARMACEUTICAL BULLETIN
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 63
- 号
- 7
- 開始ページ
- 489
- 終了ページ
- 494
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1248/cpb.c14-00869
- 出版者・発行元
- PHARMACEUTICAL SOC JAPAN
We fabricated polymeric micelles containing 5-fluorouracil (5-FU) or fluorescein using the amphiphilic block copolymer, poly-4-vinylpyridine-b-6-O-methacryloyl galactopyranose. Although the polymeric micelles were stable at pH 7.4, they readily decomposed at pH 5, resulting in near complete release of 5-FU. Uptake of polymeric micelles containing fluorescein by HepG2 and HCT116 cells was also investigated. With both cell types, strong fluorescence was observed after a 12-h incubation, but the fluorescence weakened after 24 h of incubation. The fluorescein incorporated into the polymeric micelles was released into acidic organelles (endosome and/or lysosome), from which it diffused throughout the cell. The cytotoxicity of polymeric micelles containing 5-FU was evaluated against HepG2 cells using a CCK-8 assay. The results suggest that polymeric micelles containing 5-FU are more cytotoxic to HepG2 cells than free 5-FU.
- リンク情報
- ID情報
-
- DOI : 10.1248/cpb.c14-00869
- ISSN : 0009-2363
- ORCIDのPut Code : 29638556
- Web of Science ID : WOS:000357244700002