論文

査読有り 筆頭著者
2003年5月

Role of macrophage migration inhibitory factor in otitis media with effusion in adults

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
  • Shin Kariya
  • ,
  • Mitsuhiro Okano
  • ,
  • Katsuya Aoji
  • ,
  • Michiya Kosaka
  • ,
  • Emiko Chikumoto
  • ,
  • Hisashi Hattori
  • ,
  • Koji Yuen
  • ,
  • Shinji Nishioka
  • ,
  • Keiko Nishioka
  • ,
  • Kazunori Nishizaki

10
3
開始ページ
417
終了ページ
422
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/CDLI.10.3.417-422.2003
出版者・発行元
AMER SOC MICROBIOLOGY

Otitis media with effusion (OME) is one of the most common ear diseases. Bacterial endotoxins and several inflammatory cytokines appear to be involved in the pathogenesis of OME in children; however, little is known of the immunological aspects of the onset of OME in adults. We sought to determine the presence of macrophage migration inhibitory factor (MIF) as well as interleukin 1beta (IL-1beta), tumor necrosis factor alpha (TNF-alpha), RANTES (regulated upon activation, normal T-cell expressed and presumably secreted), and endotoxin in middle ear effusions (MEEs) from adult patients with OME. In addition, the levels of MIF in MEEs from adults and children were compared. MEE was obtained from 95 adults and 11 children. The levels of MIF, IL-1beta, TNF-alpha, and RANTES were determined by enzyme-linked inummosorbent assay, and the concentrations of endotoxin and total protein were determined by the Endospec assay and bicinchoninic acid assay, respectively. MIF was detected in 97.9% of the MEEs from adults, while endotoxin, IL-1beta, TNF-alpha, and RANTES were detected in 96.8, 12.6, 5.3, and 43.9%, respectively. In addition, the level of MIF was significantly higher than those of endotoxin, IL-1beta, and TNF-alpha. A positive correlation between the levels of MIF and endotoxin was observed. MIF and endotoxin were detected in 81.8 and 72.7%, respectively, of the MEEs from the children. The level of MIF was significantly higher in the children, and conversely that of endotoxin was significantly higher in the adults. These results suggest that the interaction between MIF and endotoxin may promote fluid collection in the middle ear, particularly in adults.

リンク情報
DOI
https://doi.org/10.1128/CDLI.10.3.417-422.2003
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12738641
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000183022900015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1128/CDLI.10.3.417-422.2003
  • ISSN : 1071-412X
  • PubMed ID : 12738641
  • Web of Science ID : WOS:000183022900015

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