2018年
Application of single prolonged stress induces post-traumatic stress disorder-like characteristics in mice
Acta Medica Okayama
- ,
- ,
- ,
- 巻
- 72
- 号
- 5
- 開始ページ
- 479
- 終了ページ
- 486
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.18926/AMO/56245
© 2018, by Okayama University Medical School. We tried to clarify the applicability of a single prolonged stress (SPS) protocol as post-traumatic stress disorder (PTSD) model in mice. To investigate PTSD pathophysiology, we conducted hypothalamo-pituitary-adrenal (HPA) negative feedback testing at 1, 4, 8 and 12 weeks after the SPS by administrating a dexamethasone (DEX) suppression test. The SPS induced over-suppression of the HPA system by DEX treatment at 8 and 12 weeks. To investigate PTSD-like behavioral characteristics, we subjected mice to testing in a light/dark box (to assess anxiety), a Y-maze (working memory), a cliff avoidance (visual cognition), and an open field (locomotor activity) at 1, 4, 8 and 12 weeks after the SPS. In the light/dark box test, the SPS-applied mice spent significantly less time in the light box at 8 or 12 weeks. In the cliff avoidance test, the SPS-applied mice spent significantly less time in the open area at 1 week. However, in both the Y-maze test and the open field test, SPS-applied mice tended toward slight decreases in a time-dependent manner until 12 weeks. Therefore, SPS-applied mice may thus be useful for assessing characteristics relevant to PTSD that coincide with changes in the HPA axis.
- リンク情報
- ID情報
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- DOI : 10.18926/AMO/56245
- ISSN : 0386-300X
- PubMed ID : 30369604
- SCOPUS ID : 85055614688