論文

査読有り
2014年2月

Locally Injected Dexmedetomidine Inhibits Carrageenin-Induced Inflammatory Responses in the Injected Region

ANESTHESIA AND ANALGESIA
  • Shintaro Sukegawa
  • ,
  • Hitoshi Higuchi
  • ,
  • Miho Inoue
  • ,
  • Hitoshi Nagatsuka
  • ,
  • Shigeru Maeda
  • ,
  • Takuya Miyawaki

118
2
開始ページ
473
終了ページ
480
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1213/ANE.0000000000000060
出版者・発行元
LIPPINCOTT WILLIAMS & WILKINS

BACKGROUND: Dexmedetomidine, a highly selective agonist of 2-adrenoceptors, is a commonly used sedative; however, a potent anti-inflammatory effect has also been found. In the present study we evaluated the inhibitory effect of locally injected dexmedetomidine on inflammatory responses in the injected region.
METHODS: Local inflammation was induced in the hindpaws of male mice (aged 6-8 weeks) by intraplantar injection of lambda-carrageenin. To offset the central effect of tested agents, different agents were blindly injected into the left and right paws in the pairs of comparison. The effect of dexmedetomidine on edema (increase in paw volume), the accumulation of leukocytes, and production of tumor necrosis factor- (TNF-) and cyclooxygenase-2 (COX-2) were evaluated after carrageenin injection, using water displacement plethysmometry, histological imaging, immunohistochemistry, and Western blotting analysis. Furthermore, we also evaluated the effect of yohimbine, a full antagonist of 2-adrenoceptors, and phenylephrine, an agonist of the 1-adrenoceptor, on dexmedetomidine's action on inflammatory responses.
RESULTS: Paw volume and amount of leukocytes in the injected region significantly increased after the injection of carrageenin. Similarly, TNF- and COX-2 production was found in the subcutaneous region injected with carrageenin, 4 hours after injection. Dexmedetomidine significantly inhibited all increases in paw volume, leukocytes, and production of TNF- and COX-2. Furthermore, yohimbine significantly antagonized the anti-inflammatory effects of dexmedetomidine, whereas phenylephrine did not significantly alter them.
CONCLUSIONS: The findings suggest that locally injected dexmedetomidine exhibits an anti-inflammatory effect against local acute inflammatory responses, mediated by 2-adrenoceptors.

Web of Science ® 被引用回数 : 28

リンク情報
DOI
https://doi.org/10.1213/ANE.0000000000000060
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24445644
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000335395300030&DestApp=WOS_CPL

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