論文

査読有り
2012年4月

Effects of midazolam and phenobarbital on brain oxidative reactions induced by pentylenetetrazole in a convulsion model

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
  • Yukiko Arai
  • ,
  • Shigeru Maeda
  • ,
  • Hitoshi Higuchi
  • ,
  • Yumiko Tomoyasu
  • ,
  • Masahiko Shimada
  • ,
  • Takuya Miyawaki

34
2
開始ページ
216
終了ページ
221
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3109/08923973.2011.595417
出版者・発行元
TAYLOR & FRANCIS LTD

Context: Brain oxidative reactions are involved in epilepsy as well as neurodegenerative diseases. In animal convulsion models, some anticonvulsants have been found to suppress oxidative reactions associated with convulsions. However, the effect of anticonvulsants on brain oxidative reactions has not fully been clarified.
Objective: Midazolam and phenobarbital are often used as an intravenous anesthetic, and are known to have anticonvulsive effect, but antioxidative effect of these drugs has rarely been studied. Thus, the purpose of this study was to evaluate the effects of these drugs on the degree of convulsions and brain oxidative reactions in an animal convulsion model.
Materials and methods: In order to evaluate brain oxidative reactions, we measured malondialdehyde (MDA) level and heme oxygenase (HO)-1 mRNA expression level in the brain of mice in a convulsion model generated by a single injection of pentylenetetrazole (PTZ). We evaluated the effects of midazolam and phenobarbital on the degree of PTZ-induced convulsions and on the changes in brain MDA level and HO-1 mRNA expression level.
Results: After PTZ injection, severe convulsions were observed in all mice. MDA level was increased in the whole brain, while HO-1 mRNA expression level was increased only in the hippocampus. Both midazolam and phenobarbital prevented the convulsions and suppressed the increase in both MDA level and HO-1 mRNA expression level in the brain.
Conclusion: In this study, both midazolam and phenobarbital suppressed PTZ-induced MDA and HO-1 reactions in the brain, suggesting that these drugs inhibit brain oxidative reactions in a convulsion model.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.3109/08923973.2011.595417
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21851322
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000300444800007&DestApp=WOS_CPL

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