2005年12月
Evidence for the hydrophobic cavity of heme oxygenase-1 to be a CO-trapping site
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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- ,
- ,
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- 巻
- 338
- 号
- 1
- 開始ページ
- 584
- 終了ページ
- 589
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bbrc.2005.08.045
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Carbon monoxide (CO) is produced during the heme catabolism by heme oxygenase. In brain or blood vessels, CO functions as a neurotransmitter or an endothelial-derived relaxing factor. To verify whether crystallographically proposed CO-trapping sites of rat and cyanobacterial heme oxygenase-1 really work, heme catabolism by heme oxygenase-1 from rat and cyanobacterial Synechocystis sp. PCC 6803 has been scrutinized in the presence of 2-propanol. If 2-propanol occupies the trapping sites, formation of CO-bound verdoheme should be enhanced. Although effects of 2-propanol on the rat heme oxygenase-1 reaction were obscure, the reaction of cyanobacterial enzyme in the presence of NADPH/ferredoxin reductase/ferredoxin was apparently affected. Relative amount of CO-verdoheme versus CO-free verdoheme detected by optical absorption spectra increased as the equivalent of 2-propanol increased, thereby supporting indirectly that the hydrophobic cavity in cyanobacterial enzyme traps CO to reduce CO inhibition of verdoheme degradation. (c) 2005 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bbrc.2005.08.045
- ISSN : 0006-291X
- Web of Science ID : WOS:000233296700081