2014年5月
Bovine κ-casein inhibits human rotavirus (HRV) infection via direct binding of glycans to HRV
Journal of Dairy Science
- 巻
- 97
- 号
- 5
- 開始ページ
- 2653
- 終了ページ
- 2661
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.3168/jds.2013-7792
Human rotavirus (HRV) is a major etiologic agent of severe infantile gastroenteritis. κ-Casein (κ-CN) from both human and bovine mature milk has been reported to have anti-HRV activity; however, the mechanism of this activity is poorly understood. The present study examined the molecular basis for the protective effect of bovine κ-CN derived from late colostrum (6-7 d after parturition) and from mature milk. Among the components of casein, κ-CN is the only glycosylated protein that has been identified. Therefore, we investigated whether the glycan residues in κ-CN were involved in the anti-HRV activity. Desialylated CN obtained by neuraminidase treatment exhibited anti-HRV activity, whereas deglycosylated CN obtained by o-glycosidase treatment lacked antiviral activity, indicating that glycans were responsible for the antiviral activity of CN. Furthermore, an evanescent-field fluorescence-assisted assay showed that HRV particles directly bound to heated casein (at 95°C for 30. min) in a viral titer-dependent manner. Although the heated κ-CN retained inhibitory activity in a neutralization assay, the activity was weaker than that observed before heat treatment. Our findings i
- リンク情報
-
- DOI
- https://doi.org/10.3168/jds.2013-7792
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000334655600013&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84899121006&origin=inward
- ID情報
-
- DOI : 10.3168/jds.2013-7792
- ISSN : 0022-0302
- eISSN : 1525-3198
- Web of Science ID : WOS:000334655600013