2006年3月
Peroxisome proliferator-activated receptor subtypes differentially cooperate with other transcription factors in selective transactivation of the perilipin/PEX11 alpha gene pair
JOURNAL OF BIOCHEMISTRY
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- 巻
- 139
- 号
- 3
- 開始ページ
- 563
- 終了ページ
- 573
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1093/jb/mvj053
- 出版者・発行元
- JAPANESE BIOCHEMICAL SOC
Perilipin is an adipocyte-specific protein associated with lipid droplets that is crucial for the regulation of storage and mobilization of lipids. We earlier reported that the mouse perilipin gene is regulated by peroxisome proliferator-activated receptor (PPAR) gamma through a peroxisome proliferator-response element (PPRE) positioned upstream of the perilipin promoter. Moreover, we showed that this PPRE also controls expression of the PEX11 alpha gene, which is located further upstream. We show here that three elements, A, B, and C, in close proximity downstream of the PPRE, are essential for transactivation of the perilipin gene by PPAR gamma. Electrophoretic gel-mobility shift assays demonstrated that nuclear factor (NF)-1 subtypes bind specifically to element B. Furthermore, chromatin immunoprecipitation using 3T3-L1 cells revealed that NF-1A and NF-1B bind to element B in a differentiation-dependent fashion, whereas binding is constitutive with NF-1C and NF-1X. Element C is likely to be a binding motif for nuclear receptors. With PPAR alpha, elements A-C do not appear to be required for transactivation of the PEX11 alpha gene, so that cooperation with other transcription factors may be differentially involved in selective transactivation of the PEX11 alpha and perilipin genes by different PPAR subtypes.
- リンク情報
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- DOI
- https://doi.org/10.1093/jb/mvj053
- CiNii Articles
- http://ci.nii.ac.jp/naid/10018847055
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16567422
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000237355200028&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1093/jb/mvj053
- ISSN : 0021-924X
- CiNii Articles ID : 10018847055
- PubMed ID : 16567422
- Web of Science ID : WOS:000237355200028