論文

査読有り 責任著者 国際誌
2021年2月4日

Effects of advanced glycation end products on dental pulp calcification.

Oral diseases
  • Keita Sugiyama
  • ,
  • Jiro Miura
  • ,
  • Masato Shimizu
  • ,
  • Aoi Takashima
  • ,
  • Yusuke Matsuda
  • ,
  • Hiroki Kayashima
  • ,
  • Motoki Okamoto
  • ,
  • Tadashi Nagashima
  • ,
  • Tsutomu Araki

記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/odi.13792

OBJECTIVE: The main aim of this study was to elucidate the effects of advanced glycation end products (AGEs) on the calcification of cultured rat dental pulp cells (RDPCs) and to investigate the crystallisation ability of glycated collagen. MATERIALS AND METHODS: AGEs were prepared via non-enzymatic glycation of a dish coated with type I collagen using dl-glyceraldehyde. To investigate the effects of AGEs on RDPCs, we performed WST-1 and lactate dehydrogenase assays; alkaline phosphatase, Alizarin Red S and immunohistochemical staining; and real-time quantitative reverse transcription PCR. In addition, we performed crystallisation experiments on glycated collagen. All microstructures were analysed using scanning electron microscopy/energy-dispersive X-ray spectroscopy and transmission electron microscopy/diffraction pattern analysis. RESULTS: AGEs did not affect the proliferation or differentiation of RDPCs, but enhanced the calcification rate and cytotoxicity. No major calcification-related genes or proteins were involved in these calcifications, and glycated collagen was found to exhibit a negative polarity and form calcium phosphate crystals. Cytotoxicity due to drastic changes in the concentration of pericellular ions led to dystrophic calcification, assumed to represent an aspect of diabetic pulp calcifications. CONCLUSION: Glycated collagen-containing AGEs provide a nurturing environment for crystallisation and have a significant effect on the early calcification of RDPCs.

リンク情報
DOI
https://doi.org/10.1111/odi.13792
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33539626
ID情報
  • DOI : 10.1111/odi.13792
  • PubMed ID : 33539626

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