1998年2月
Isolation of differentially expressed genes upon immunoglobulin class switching by a subtractive hybridization method using uracil DNA glycosylase
NUCLEIC ACIDS RESEARCH
- ,
- ,
- ,
- 巻
- 26
- 号
- 4
- 開始ページ
- 911
- 終了ページ
- 918
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/nar/26.4.911
- 出版者・発行元
- OXFORD UNIV PRESS
Immunoglobulin class switch recombination enables B lymphocytes to sequentially express antibodies that have identical specificities but that differ in class and effector function, Although several cis elements required for class switch recombination have been identified, few trans-acting factors which are directly related to class switching have been found, Previously we have developed an efficient in vitro class switching system using a cell line, CH12F3-2. To clarify the molecular mechanism of class switching, we intended to isolate genes induced with interleukin (IL)-4, transforming growth factor (TGF)-beta and CD40L using the in vitro class switching system, For that purpose, an improved method for making subtracted cDNA libraries, using uracil DNA glycosylase, has been developed, This method can overcome a general problem of subtraction, that rare cDNAs are easily lost, This new subtraction method was applied to the CH12F3-2 switching system to isolate genes induced by stimulations with IL-4, TGF-beta and CD40L, and cDNAs encoding deiodinase 1 and SS32, an alternatively spliced form of the muscle LIM protein, were obtained, Their expression patterns in response to various combinations of stimuli and the time courses of the induction supported the usefulness of this method.
- リンク情報
- ID情報
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- DOI : 10.1093/nar/26.4.911
- ISSN : 0305-1048
- PubMed ID : 9461447
- Web of Science ID : WOS:000072236300006