論文

査読有り 国際誌
2019年10月

Label-free real-time imaging of extracellular Ca2+ uptake in the hippocampal slice using Ca-PVC membrane based on charge-transfer-type potentiometric sensor arrays

Proceedings of IEEE Sensors
  • Hideo Doi
  • ,
  • Kazuaki Sawada
  • ,
  • Tomoko Horio
  • ,
  • Eiji Shigetomi
  • ,
  • Bijay Parajuli
  • ,
  • Youichi Shinozaki
  • ,
  • Toshihiko Noda
  • ,
  • Kazuhiro Takahashi
  • ,
  • Toshiaki Hattori
  • ,
  • Schuichi Koizumi

2019-October
記述言語
英語
掲載種別
研究論文(国際会議プロシーディングス)
DOI
10.1109/SENSORS43011.2019.8956934

© 2019 IEEE. An elevation of intracellular Ca2+ concentration during neuronal excitation is widely known, and much research has already been done. However, the changes in extracellular Ca2+ concentration ([Ca2+]o) have received only limited attention, and its imaging and spatiotemporal analysis has not been achieved. Thus, we develop a [Ca2+]o imaging sensor to clarify the role(s) of [Ca2+]o in regulation of physiological and pathophysiological neurons. 23.55-μm-pitch and 128×128 pixels Ca2+ image sensor using plasticized polyvinyl chloride (PVC) as an ion sensitive membrane was fabricated and the diffusion image of Ca2+ concentration in sample solution was successfully obtained. The sensitivity was 23.3 mV/decade. The mouse hippocampal slice was placed on the sensor, and stimulated with glutamate and its related agonists. When stimulated, the output image was clearly changed, i.e., glutamate decreased [Ca2+]o via NMDA receptor. We also found that such [Ca2+]o changes were obvious in CA3 and dentate gyrus (DG) of the hippocampus, and were not observed in the slice-free sensor area. Taken together, we succeeded in the development of the [Ca2+]o imaging device, allowing us to monitor and analyze real-time imaging of the spatiotemporal changes in [Ca2+]o in the brain slices.

リンク情報
DOI
https://doi.org/10.1109/SENSORS43011.2019.8956934
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85078702633&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85078702633&origin=inward
ID情報
  • DOI : 10.1109/SENSORS43011.2019.8956934
  • ISSN : 1930-0395
  • eISSN : 2168-9229
  • SCOPUS ID : 85078702633

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