2019年10月
Label-free real-time imaging of extracellular Ca2+ uptake in the hippocampal slice using Ca-PVC membrane based on charge-transfer-type potentiometric sensor arrays
Proceedings of IEEE Sensors
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- 巻
- 2019-October
- 号
- 記述言語
- 英語
- 掲載種別
- 研究論文(国際会議プロシーディングス)
- DOI
- 10.1109/SENSORS43011.2019.8956934
© 2019 IEEE. An elevation of intracellular Ca2+ concentration during neuronal excitation is widely known, and much research has already been done. However, the changes in extracellular Ca2+ concentration ([Ca2+]o) have received only limited attention, and its imaging and spatiotemporal analysis has not been achieved. Thus, we develop a [Ca2+]o imaging sensor to clarify the role(s) of [Ca2+]o in regulation of physiological and pathophysiological neurons. 23.55-μm-pitch and 128×128 pixels Ca2+ image sensor using plasticized polyvinyl chloride (PVC) as an ion sensitive membrane was fabricated and the diffusion image of Ca2+ concentration in sample solution was successfully obtained. The sensitivity was 23.3 mV/decade. The mouse hippocampal slice was placed on the sensor, and stimulated with glutamate and its related agonists. When stimulated, the output image was clearly changed, i.e., glutamate decreased [Ca2+]o via NMDA receptor. We also found that such [Ca2+]o changes were obvious in CA3 and dentate gyrus (DG) of the hippocampus, and were not observed in the slice-free sensor area. Taken together, we succeeded in the development of the [Ca2+]o imaging device, allowing us to monitor and analyze real-time imaging of the spatiotemporal changes in [Ca2+]o in the brain slices.
- リンク情報
- ID情報
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- DOI : 10.1109/SENSORS43011.2019.8956934
- ISSN : 1930-0395
- eISSN : 2168-9229
- SCOPUS ID : 85078702633