論文

2018年10月

Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics

Genes & development
  • Horie, Takeo
  • ,
  • Horie, Ryoko
  • ,
  • Chen, Kai
  • ,
  • Cao, Chen
  • ,
  • Nakagawa, Masashi
  • ,
  • Kusakabe
  • ,
  • Takehiro G
  • ,
  • Satoh, Noriyuki
  • ,
  • Sasakura, Yasunori
  • ,
  • Levine, Michael

32
19-20
開始ページ
1297
終了ページ
1302
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1101/gad.317669.118
出版者・発行元
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT

The CNS of the protovertebrate contains a single cluster of dopaminergic (DA) neurons, the coronet cells, which have been likened to the hypothalamus of vertebrates. Whole-embryo single-cell RNA sequencing (RNA-seq) assays identified as the most strongly expressed cell-specific transcription factor (TF) in DA/coronet cells. Knockdown of activity results in their loss, while misexpression results in the appearance of supernumerary DA/coronet cells. Photoreceptor cells and ependymal cells are the most susceptible to transformation, and both cell types express high levels of Coexpression of both and caused the wholesale transformation of the entire CNS into DA/coronet cells. We therefore suggest that the reiterative use of functional manipulations and single-cell RNA-seq assays is an effective means for the identification of regulatory cocktails underlying the specification of specific cell identities.

リンク情報
DOI
https://doi.org/10.1101/gad.317669.118
ID情報
  • DOI : 10.1101/gad.317669.118
  • ISSN : 1549-5477

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