We show vicariance of Cicindela chinensis in Okinawa, Japan (differentiated within Japan) and Korea-China through construction of Bayesian inference trees by BEAST2. Calibration was done using an assumption of the MRCA expansion of C.chinensis at 1.55Ma (=geologically obtained formative time of the Ryukyu islands) following the protocol of BEAUti. We derived substitution rates for mitochondrial COI (1.66%/m.y.) and nuclear 28SrRNA (0.109%/m.y.) of analyzed Cicindela. Cicindela ferriei is a sister of C.chinensis, and these two species differentiated from each other at ca. 3Ma before the expansion of C.chinensis. However, they are not strongly differentiated between Amami-Oshima and Tokuno-shima, although they display different color. Vicariance at 1.55Ma is also recognized between Cicindela batesi in Taiwan and Cicindela aurulenta and virgula in continental China. From the sequence data we obtained, it is also evident that C.c.okinawana recently colonized Ishigaki-jima from Okinawa-jima, as did C.batesi in Iriomote-jima from Taiwan.