MIYASHITA Masahiro

Last updated: Oct 30, 2018 at 15:29

Name	MIYASHITA Masahiro
Affiliation	Kyoto University
Section	Graduate School of Agriculture, Division of Applied Life Sciences
Degree	Doctor of Agricultural Science(Kyoto University)

Research Interests

Synthesis and Structure-Activity Relationship Study of Bioactive Peptide(1)

Research Areas

Agricultural chemistry / Bioproduction chemistry/Bioorganic chemistry /

Academic & Professional Experience

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1999

2000

Postdoctral Researcher University of California,

Davis

Education

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1998

Department of Agricultural Chemistry, Graduate School, Division of Agriculture, Kyoto University

1993

Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

Published Papers

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Fundamental and Practical Aspects of de novo Peptide Sequencing

MIYASHITA Masahiro

J. Mass Spectrom. Soc. Japan 65 231-238 2017

Complete de novo sequencing of antimicrobial peptides in the venom of the scorpion Isometrus maculatus

Miyashita, M. Kitanaka, A. Yakio, M. Yamazaki, Y. Nakagawa, Y. Miyagawa, H.

Toxicon 139 1-12 2017 [Refereed]

A Facile Method for Preferential Modification of the N-Terminal Amino Group of Peptides Using Triazine-Based Coupling Reagents

Kitanaka, A. Juichi, H. Nihashi, Y. Miyashita, M. Miyagawa, H.

Mass Spectrom (Tokyo) 6(1) A0059 2017 [Refereed]

Isolation, structural identification and biological characterization of two conopeptides from the Conus pennaceus venom

Abdel-Wahab, M. Miyashita, M. Ota, Y. Juichi, H. Okabe, R. Sarhan, M. Fouda, M. Abdel-Rahman, M. Saber, S. Nakagawa, Y.

Biosci Biotechnol Biochem 81 2086-2089 2017 [Refereed]

Early signaling events induced by the peptide elicitor PIP-1 necessary for acetosyringone accumulation in tobacco cells.

Kim Y, Miyashita M, Miyagawa H

Biosci Biotechnol Biochem 80(6) 1057 Jun 2016 [Refereed]

Characterization of the venom of the vermivorous cone snail Conus fulgetrum.

Abdel-Wahab M, Miyashita M, Kitanaka A, Juichi H, Sarhan M, Fouda M, Abdel-Rahman M, Saber S, Nakagawa Y

Biosci Biotechnol Biochem 80(10) 1882 Apr 2016 [Refereed]

N-Terminal Derivatization with Structures Having High Proton Affinity for Discrimination between Leu and Ile Residues in Peptides by High-Energy Collision-Induced Dissociation

Kitanaka, A. Miyashita, M. Kubo, A. Satoh, T. Toyoda, M. Miyagawa, H.

Mass Spectrom (Tokyo) 5 A0051 2016 [Refereed]

Chemical synthesis of La1 isolated from the venom of the scorpion Liocheles australasiae and determination of its disulfide bonding pattern.

Nagao J, Miyashita M, Nakagawa Y, Miyagawa H

J. Pept. Sci. 21(8) 636-643 Aug 2015 [Refereed]

Photocontrol of Elicitor Activity of PIP-1 to Investigate Temporal Factors Involved in Phytoalexin Biosynthesis.

Kim Y, Miyashita M, Miyagawa H

J Agric Food Chem 63(25) 5894-5901 Jul 2015 [Refereed]

Crystallization and preliminary X-ray diffraction studies of La1 from Liocheles australasiae

S. Kamachi, J. Nagao, M. Miyashita, Y. Nakagawa, H. Miyagawa, and T. Tada

Acta crystallographica. Section F, Structural biology communications 70 915-917 2014 [Refereed]

Winners of CASMI2013: Automated Tools and Challenge Data

T. Nishioka, T. Kasama, T. Kinumi, H. Makabe, F. Matsuda, D. Miura, M. Miyashita, T. Nakamura, K. Tanaka, and A. Yamamoto

Mass Spectrometry 3 S0039 2014 [Refereed]

Continuous Stimulation of the Plant Immune System by the Peptide Elicitor PIP-1 Is Required for Phytoalexin Biosynthesis in Tobacco Cells

Y. Kim, E. Komoda, M. Miyashita, and H. Miyagawa

J Agr Food Chem 62 5781-5788 2014 [Refereed]

Isolation and characterization of an anti-insect β-Toxin from the venom of the scorpion Isometrus maculatus

T. Kawachi; M. Miyashita; Y. Nakagawa; H. Miyagawa

Bioscience, Biotechnology and Biochemistry 77(1) 205-207 Jan 2013 [Refereed]

Differential 14N/15N-Labeling of Peptides Using N-Terminal Charge Derivatization with a High-Proton Affinity for Straightforward de novo Peptide Sequencing

Y. Nihashi, M. Miyashita, H. Awane, and H. Miyagawa

Mass Spectrometry 2 A0024 2013 [Refereed]

Isolation and characterization of a novel non-selective β-toxin from the venom of the scorpion isometrus maculatus

Y. Ichiki; T. Kawachi; M. Miyashita; Y. Nakagawa; H. Miyagawa

Bioscience, Biotechnology and Biochemistry 76(11) 2089-2092 Nov 2012 [Refereed]

LC/MS/MS identification of 20-hydroxyecdysone in a scorpion (Liocheles australasiae) and its binding affinity to in vitro-translated molting hormone receptors

M. Miyashita; K. Matsushita; S. Nakamura; S. Akahane; Y. Nakagawa; H. Miyagawa

Insect Biochemistry and Molecular Biology 41(12) 932-937 Dec 2011 [Refereed]

Solution structure of a short-chain insecticidal toxin LaIT1 from the venom of scorpion Liocheles australasiae

S. Horita; N. Matsushita; T. Kawachi; R. Ayabe; M. Miyashita; T. Miyakawa; Y. Nakagawa; K. Nagata; H. Miyagawa; M. Tanokura

Biochemical and Biophysical Research Communications 411(4) 738-744 Aug 2011 [Refereed]

Discovery of a Small Peptide from Combinatorial Libraries That Can Activate the Plant Immune System by a Jasmonic Acid Signaling Pathway

M. Miyashita; M. Oda; Y. Ono; E. Komoda; H. Miyagawa

ChemBioChem 12(9) 1323-1329 Jun 2011 [Refereed]

Improving peptide fragmentation by N-terminal derivatization with high proton affinity

M. Miyashita; Y. Hanai; H. Awane; T. Yoshikawa; H. Miyagawa

Rapid Communications in Mass Spectrometry 25(9) 1130-1140 2011 [Refereed]

A novel amphipathic linear peptide with both insect toxicity and antimicrobial activity from the venom of the scorpion isometrus maculatus

M. Miyashita; A. Sakai; N. Matsushita; Y. Hanai; Y. Nakagawa; H. Miyagawa

Bioscience, Biotechnology and Biochemistry 74(2) 364-369 Feb 2010 [Refereed]

Studies on bioorganic chemistry of peptides active against insects and plants

M. Miyashita

Journal of Pesticide Science 35(4) 496-498 2010

Purification and cDNA cloning of LaIT2, a novel insecticidal toxin from venom of the scorpion Liocheles australasiae

N. Matsushita; M. Miyashita; Y. Ichiki; T. Ogura; E. Sakuradani; Y. Nakagawa; S. Shimizu; H. Miyagawa

Bioscience, Biotechnology and Biochemistry 73(12) 2769-2772 Dec 2009 [Refereed]

Cellular internalization of arginine-rich peptides into tobacco suspension cells: A structure-activity relationship study

T. Mizuno; M. Miyashita; H. Miyagawa

Journal of Peptide Science 15(4) 259-263 Apr 2009 [Refereed]

Plant pathogen recognition as a natural, original and simple model for chemogenomics: A brief overview of cell-based assays to screen for pep tides acting as plant defense activators

M. Miyashita; H. Miyagawa

Combinatorial Chemistry and High Throughput Screening 11(8) 647-652 Sep 2008 [Refereed]

LPT1 encodes a membrane-bound O-acyltransferase involved in the acylation of lysophospholipids in the yeast Saccharomyces cerevisiae

H. Tamaki; A. Shimada; Y. Ito; M. Ohya; J. Takase; M. Miyashita; H. Miyagawa; H. Nozaki; R. Nakayama; H. Kumagai

Journal of Biological Chemistry 282(47) 34288-34298 2007 [Refereed]

Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae

Y. Nakagawa; A. Sakai; F. Magata; T. Ogura; M. Miyashita; H. Miyagawa

FEBS Journal 274(23) 6191-6203 2007 [Refereed]

Purification and characterization of a novel short-chain insecticidal toxin with two disulfide bridges from the venom of the scorpion Liocheles australasiae

N. Matsushita; M. Miyashita; A. Sakai; Y. Nakagawa; H. Miyagawa

Toxicon 50(6) 861-867 2007 [Refereed]

Characterization of peptide components in the venom of the scorpion Liocheles australasiae (Hemiscorpiidae)

M. Miyashita; J. Otsuki; Y. Hanai; Y. Nakagawa; H. Miyagawa

Toxicon 50(3) 428-437 2007 [Refereed]

Development of a high-throughput screening method using a cell-based, lawn format assay for the identification of novel plant defense activators from combinatorial peptide libraries

M. Miyashita; Y. Otake; M. Oda; H. Miyagawa

Journal of Agricultural and Food Chemistry 55(3) 806-811 2007 [Refereed]

Development of a colorimetric assay for determining the amount of H ₂O₂ generated in tobacco cells in response to elicitors and its application to study of the structure-activity relationship of flagellin-derived peptides

K.-I. Miyata; M. Miyashita; R. Nose; Y. Otake; H. Miyagawa

Bioscience, Biotechnology and Biochemistry 70(9) 2138-2144 2006 [Refereed]

Surface plasmon resonance-based immunoassay for 17β-estradiol and its application to the measurement of estrogen receptor-binding activity

M. Miyashita; T. Shimada; H. Miyagawa; M. Akamatsu

Analytical and Bioanalytical Chemistry 381(3) 667-673 Feb 2005 [Refereed]

Determination of potato glycoalkaloids using high-pressure liquid chromatography-electrospray ionisation/mass spectrometry

F. Matsuda; K. Morino; H. Miyazawa; M. Miyashita; H. Miyagawa

Phytochemical Analysis 15(2) 121-124 Mar 2004 [Refereed]

Enantioselective recognition of mono-demethylated methoxychlor metabolites by the estrogen receptor

M. Miyashita; T. Shimada; S. Nakagami; N. Kurihara; H. Miyagawa; M. Akamatsu

Chemosphere 54(8) 1273-1276 Feb 2004 [Refereed]

Metabolic flux analysis of the phenylpropanoid pathway in wound-healing potato tuber tissue using stable isotope-labeled tracer and LC-MS spectroscopy

F. Matsuda; K. Morino; M. Miyashita; H. Miyagawa

Plant and Cell Physiology 44(5) 510-517 May 2003 [Refereed]

Inhibitory activity of analogs of AM-toxin, a host-specific phytotoxin from the Alternaria alternata apple pathotype, on photosynthetic O₂ evolution in apple leaves

M. Miyashita; T. Nakamori; H. Miyagawa; M. Akamatsu; T. Ueno

Bioscience, Biotechnology and Biochemistry 67(3) 635-638 Mar 2003 [Refereed]

Structure-activity relationship study of host-specific phytotoxins (AM-toxin analogs) using a new assay method with leaves from apple meristem culture

M. Miyashita; T. Nakamori; T. Murai; T. Yonemoto; H. Miyagawa; M. Akamatsu; T. Ueno

Zeitschrift fur Naturforschung - Section C Journal of Biosciences 56(11-12) 1029-1037 Nov 2001 [Refereed]

Attomole level protein sequencing by Edman degradation coupled with accelerator mass spectrometry

M. Miyashita; J.M. Presley; B.A. Buchholz; K.S. Lam; Y.M. Lee; J.S. Vogel; B.D. Hammock

Proceedings of the National Academy of Sciences of the United States of America 98(8) 4403-4408 2001 [Refereed]

Three-dimensional quantitative structure-activity relationship analyses of RGD mimetics as fibrinogen receptor antagonists

M. Miyashita; M. Akamatsu; Y. Hayashi; T. Ueno

Bioorganic and Medicinal Chemistry Letters 10(9) 859-863 May 2000 [Refereed]

Structure-activity relationships of RGD mimetics as fibrinogen-receptor antagonists

M. Miyashita; M. Akamatsu; H. Ueno; Y. Nakagawa; K. Nishimura; Y. Hayashi; Y. Sato; T. Ueno

Bioscience, Biotechnology and Biochemistry 63(10) 1684-1690 Oct 1999 [Refereed]

Misc

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Isolation and Characterization of a Short-Chain Insecticidal Toxin LaIT1 from the Venom of the Scorpion Liocheles australasiae

Masahiro Miyashita

4th Pan Pacific Conference on Pesticide Science 2008 [Refereed]

Novel Peptide Elicitor Identified from Combinatorial Random Peptide Libraries

Masahiro Miyashita

4th Pan Pacific Conference on Pesticide Science 2008 [Refereed]

Cellular Internalization of Arginine-Rich Peptides into Plant Cells

Masahiro Miyashita

4th Pan Pacific Conference on Pesticide Science 2008 [Refereed]

Structure-activity relationship study of a novel hexapeptide that can activate the plant immune system

Masahiro Miyashita

1th China-Japan-Korea Workshop on Pesticide Science 2009 [Refereed]

Novel linear pep,de with both insect toxicity and an,microbial actvity from the venom of the scorpion Isometrus maculatus

Masahiro Miyashita

1st China-Japan-Korea Workshop on Pesticide Science 2009 [Refereed]

Conference Activities & Talks

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Search for new insecticidal peptide from the venom of the North African scorpion, Buthacus leptochelys.

Yoshimoto, Y., Abdel-Wahab, M., Sarhan, M., Miyashita, M., Nakagawa, Y., Miyagawa, H

17 Mar 2018

Analysis of protein expression in tobacco cell wall induced by elicitor treatment

NAKAMURA, Y., MIYASHITA, M., MIYAGAWA, H.,

16 Mar 2018

A facile method for preferential modification of the N-terminal amino group of peptides using DMT- MM

Hironori Juichi, Atsushi Kitanaka, Yoichiro Nihashi, Masahiro Miyashita, Hisashi Miyagawa

Nov 2017

Chemical modification-assisted de novo sequencing of antimicrobial peptides in the scorpion venom

Masahiro Miyashita, Atsushi Kitanaka, Mao Yakio, Yuzo Yamazaki, Yoshiaki Nakagawa, Hisashi Miyagawa

IST2017 World Congress Oct 2017

Exploration and indentification of the antimicrobial peptides from the venom of the scorpion Liocheles australasiae

OKABE, R., KITANAKA, A., UCHIYAMA, H., MIYASHITA, M., NAKAGAWA, Y., MIYAGAWA, H.

20 Mar 2017

Research Grants & Projects

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Synthesis and Activity of Bioactive Peptides

Project Year: 2000