2007年11月
Increased formation of hepatic N-2-ethylidene-2'-deoxyguanosine DNA adducts in aldehyde dehydrogenase 2-knockout mice treated with ethanol
CARCINOGENESIS
- 巻
- 28
- 号
- 11
- 開始ページ
- 2363
- 終了ページ
- 2366
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/carcin/bgm057
- 出版者・発行元
- OXFORD UNIV PRESS
N-2-ethylidene-2'-deoxyguanosine (N-2-ethylidene-dG) is a major DNA adduct induced by acetaldehyde. Although it is unstable in the nucleoside form, it is relatively stable when present in DNA. In this study, we analyzed three acetaldehyde-derived DNA adducts, N-2-ethylidene-dG, N-2-ethyl-2'-deoxyguanosine (N-2-Et-dG) and alpha-methyl-gamma-hydroxy-1,N-2-propano-2'-deoxyguanosine (alpha-Me-gamma-OH-PdG) in the liver DNA of aldehyde dehydrogenase (Aldh)-2-knockout mice to determine the influence of alcohol consumption and the Aldh2 genotype on the levels of DNA damage. In control Aldh2+/+ mice, the level of N-2-ethylidene-dG adduct in liver DNA was 1.9 +/- 0.7 adducts per 10(7) bases and was not significantly different than that of Aldh2+/- and -/- mice. In alcohol-fed mice (20% ethanol for 5 weeks), the adduct levels of Aldh2+/+, +/- and -/- mice were 7.9 +/- 1.8, 23.3 +/- 4.0 and 79.9 +/- 14.2 adducts per 10(7) bases, respectively, and indicated that adduct level was alcohol and Aldh2 genotype dependent. In contrast, an alcohol- or Aldh2 genotype-dependent increase was not observed for alpha-Me-gamma-OH-PdG, and N-2-Et-dG was not detected in any of the analyzed samples. In conclusion, the risk of formation of N-2-ethylidene-dG in model animal liver in vivo is significantly higher in the Aldh2-deficient population and these results may contribute to our understanding of in vivo adduct formation in humans.
- リンク情報
-
- DOI
- https://doi.org/10.1093/carcin/bgm057
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17361010
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000250826400015&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34547120224&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=34547120224&origin=inward
- ID情報
-
- DOI : 10.1093/carcin/bgm057
- ISSN : 0143-3334
- eISSN : 1460-2180
- PubMed ID : 17361010
- SCOPUS ID : 34547120224
- Web of Science ID : WOS:000250826400015