論文

査読有り 本文へのリンクあり 国際誌
2007年11月

Increased formation of hepatic N-2-ethylidene-2'-deoxyguanosine DNA adducts in aldehyde dehydrogenase 2-knockout mice treated with ethanol

CARCINOGENESIS
  • Tomonari Matsuda
  • Akiko Matsumoto
  • Mitsuhiro Uchida
  • Robert A. Kanaly
  • Kentaro Misaki
  • Shinya Shibutani
  • Toshihiro Kawamoto
  • Kyoko Kitagawa
  • Keiichi I. Nakayama
  • Katsumaro Tomokuni
  • Masayoshi Ichiba
  • 全て表示

28
11
開始ページ
2363
終了ページ
2366
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/carcin/bgm057
出版者・発行元
OXFORD UNIV PRESS

N-2-ethylidene-2'-deoxyguanosine (N-2-ethylidene-dG) is a major DNA adduct induced by acetaldehyde. Although it is unstable in the nucleoside form, it is relatively stable when present in DNA. In this study, we analyzed three acetaldehyde-derived DNA adducts, N-2-ethylidene-dG, N-2-ethyl-2'-deoxyguanosine (N-2-Et-dG) and alpha-methyl-gamma-hydroxy-1,N-2-propano-2'-deoxyguanosine (alpha-Me-gamma-OH-PdG) in the liver DNA of aldehyde dehydrogenase (Aldh)-2-knockout mice to determine the influence of alcohol consumption and the Aldh2 genotype on the levels of DNA damage. In control Aldh2+/+ mice, the level of N-2-ethylidene-dG adduct in liver DNA was 1.9 +/- 0.7 adducts per 10(7) bases and was not significantly different than that of Aldh2+/- and -/- mice. In alcohol-fed mice (20% ethanol for 5 weeks), the adduct levels of Aldh2+/+, +/- and -/- mice were 7.9 +/- 1.8, 23.3 +/- 4.0 and 79.9 +/- 14.2 adducts per 10(7) bases, respectively, and indicated that adduct level was alcohol and Aldh2 genotype dependent. In contrast, an alcohol- or Aldh2 genotype-dependent increase was not observed for alpha-Me-gamma-OH-PdG, and N-2-Et-dG was not detected in any of the analyzed samples. In conclusion, the risk of formation of N-2-ethylidene-dG in model animal liver in vivo is significantly higher in the Aldh2-deficient population and these results may contribute to our understanding of in vivo adduct formation in humans.

リンク情報
DOI
https://doi.org/10.1093/carcin/bgm057
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17361010
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000250826400015&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34547120224&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=34547120224&origin=inward
ID情報
  • DOI : 10.1093/carcin/bgm057
  • ISSN : 0143-3334
  • eISSN : 1460-2180
  • PubMed ID : 17361010
  • SCOPUS ID : 34547120224
  • Web of Science ID : WOS:000250826400015

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