論文

国際誌
2020年3月10日

Overexpression of Nuclear Receptor 5A1 Induces and Maintains an Intermediate State of Conversion between Primed and Naive Pluripotency.

Stem cell reports
  • Kaori Yamauchi
  • ,
  • Tatsuhiko Ikeda
  • ,
  • Mihoko Hosokawa
  • ,
  • Norio Nakatsuji
  • ,
  • Eihachiro Kawase
  • ,
  • Shinichiro Chuma
  • ,
  • Kouichi Hasegawa
  • ,
  • Hirofumi Suemori

14
3
開始ページ
506
終了ページ
519
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.stemcr.2020.01.012

Naive and primed human pluripotent stem cells (hPSCs) have provided useful insights into the regulation of pluripotency. However, the molecular mechanisms regulating naive conversion remain elusive. Here, we report intermediate naive conversion induced by overexpressing nuclear receptor 5A1 (NR5A1) in hPSCs. The cells displayed some naive features, such as clonogenicity, glycogen synthase kinase 3β, and mitogen-activated protein kinase (MAPK) independence, expression of naive-associated genes, and two activated X chromosomes, but lacked others, such as KLF17 expression, transforming growth factor β independence, and imprinted gene demethylation. Notably, NR5A1 negated MAPK activation by fibroblast growth factor 2, leading to cell-autonomous self-renewal independent of MAPK inhibition. These phenotypes may be associated with naive conversion, and were regulated by a DPPA2/4-dependent pathway that activates the selective expression of naive-associated genes. This study increases our understanding of the mechanisms regulating the conversion from primed to naive pluripotency.

リンク情報
DOI
https://doi.org/10.1016/j.stemcr.2020.01.012
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32084386
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066342

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