論文

査読有り
2015年10月

Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells

SCIENTIFIC REPORTS
  • Takuma Ojima
  • Eri Shibata
  • Shiho Saito
  • Masashi Toyoda
  • Hideki Nakajima
  • Mayu Yamazaki-Inoue
  • Yoshitaka Miyagawa
  • Nobutaka Kiyokawa
  • Jun-ichiro Fujimoto
  • Toshinori Sato
  • Akihiro Umezawa
  • 全て表示

5
開始ページ
14988
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep14988
出版者・発行元
NATURE PUBLISHING GROUP

Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. However, Comprehensive GSL profiles of hiPSCs have not yet been elucidated. The global images of GSLs from the parental cells, hiPSCs, and differentiated cells revealed that there are parental cell-independent specific glycolipids, including Globo H (fucosyl-Gb5Cer) and H type1 antigen (fucosyl-Lc4Cer) that are novel markers for undifferentiated hiPSCs. Interestingly, undifferentiated hiPSCs expressed H type 1 antigen, specific for blood type O, regardless of the cells' genotypes. Thus, in this study, we defined the dynamics of GSL remodeling during reprogramming from parental cell sets to iPSC sets and thence to iPSC-neural cells.

リンク情報
DOI
https://doi.org/10.1038/srep14988
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26477663
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000362989900001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/srep14988
  • ISSN : 2045-2322
  • PubMed ID : 26477663
  • Web of Science ID : WOS:000362989900001

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