2010年11月
An Alternative Mechanism for Radioprotection by Dimethyl Sulfoxide, Possible Facilitation of DNA Double-strand Break Repair
JOURNAL OF RADIATION RESEARCH
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- 巻
- 51
- 号
- 6
- 開始ページ
- 733
- 終了ページ
- 740
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1269/jrr.09106
- 出版者・発行元
- JAPAN RADIATION RESEARCH SOC
The radioprotective effects of dimethyl sulfoxide (DMSO) have been known for many years, and the suppression of hydroxyl (OH) radicals induced by ionizing radiation has been thought to be the main cause of this effect. However, the DMSO concentration used was very high, and might be toxic, in earlier studies. In the present study, we administered a lower, non-toxic concentration (0.5%, i.e., 64 mM) of DMSO before irradiation and examined its radioprotective effects. Colony formation assay and micronucleus assay showed significant radioprotective effects in CHO, but not in xrs5, which is defective in the repair function of DNA double-strand breaks. The levels of phosphorylated H2AX and the formation of 53BP1 foci 15 minutes after irradiation, which might reflect initial DNA double-strand breaks, in DMSO-treated CHO cells were similar to those in non-treated cells, suggesting that the radioprotective effects were not attributable to the suppression of general indirect action in the lower concentration of DMSO. On the other hand, 2 hours after irradiation, the average number of 53BP1 foci, which might reflect residual DNA double-strand breaks, was significantly decreased in DMSO-treated CHO cells compared to non-treated cells. The results indicated that low concentration of DMSO exerts radioprotective effects through the facilitation of DNA double-strand break repair rather than through the suppression of indirect action.
- リンク情報
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- DOI
- https://doi.org/10.1269/jrr.09106
- CiNii Articles
- http://ci.nii.ac.jp/naid/10026964239
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/21116101
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000285174400014&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1269/jrr.09106
- ISSN : 0449-3060
- CiNii Articles ID : 10026964239
- PubMed ID : 21116101
- Web of Science ID : WOS:000285174400014