2006年
Overexpression, purification, crystallization and preliminary X-ray cystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 62
- 号
- 12
- 開始ページ
- 1266
- 終了ページ
- 1268
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1107/S1744309106047543
- 出版者・発行元
- BLACKWELL PUBLISHING
To elucidate the structure and molecular mechanism of a characteristic proline-specific aminopeptidase produced by the thermophile Aneurinibacillus sp. strain AM-1, its gene was cloned and the recombinant protein was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.8 angstrom resolution from the recombinant aminopeptidase crystal. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 93.62, b = 68.20, c = 76.84 angstrom. A complete data set was also obtained from crystals of SeMet-substituted aminopeptidase. Data in the resolution range 20-2.1 angstrom from the MAD data set from the SeMet-substituted crystal were used for phase determination.
- リンク情報
-
- DOI
- https://doi.org/10.1107/S1744309106047543
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17142913
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000242429700024&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-33845484892&partnerID=MN8TOARS
- ID情報
-
- DOI : 10.1107/S1744309106047543
- ISSN : 1744-3091
- ORCIDのPut Code : 58433933
- PubMed ID : 17142913
- SCOPUS ID : 33845484892
- Web of Science ID : WOS:000242429700024